菰物种专化DNA序列的克隆及其在检测菰DNA导入水稻中的应用_英文_.pdfVIP

2000, 42 ( 3) : 324- 326 A cta Botanica Sinica Short Communication DNA DNA * 刘振兰 董玉柱 刘 宝 ( , 130024) : N RPS 2 ( NBS) ( LRR) , PCR ( Oryz a sativa L.) ( Ziz ania latfi olia ( Griseb. ) Turcz. ex Sta f) DNA PCR , DNA , , 60 2 , DNA Southern 2 _! DNA, 2 , Eco R∀ 5 Southern , 2 DNA : DNA ; ; ; : Q943. 2 : A :2000) Isolation of Zizania latfi olia Species_specific DNA Sequences and Their Utility in Identification of Z. latfi olia DNA Introgressed into Rice * LIU Zhen_Lan, DONG Yu_Zhu, LIU Bao ( I nstitute of Genetics and Cy tology , N ortheast N ormal Univers ity , Changchun 130024, China) Key words s ecies_s ecific DNA sequence; introgression lines; Ziz ania latfi olia ; rice Ziz ania latfi olia ( Griseb. ) Turcz. ex Sta f is a is to use cloned DNA sequences s ecific to the donor wild erennial grass belonging to the subtribe Ziz aniinae s ecies as hybridization robes. In the resent a er, we of tribe Oryz eae , widely growing in North America and re ort the isolation of two clones from genomic DNA of [ 1] East Asia . This wild s ecies ossesses numerous traits Z latfi olia that are exclusively s ecific