2个甜菜夜蛾信息素结合蛋白cDNA片段的克隆和序列分析.pdfVIP

中国农业科学 2005,38(7):1501-1504 Scientia Agricultura Sinica 2个甜菜夜蛾信息素结合蛋白cDNA片段的克隆和序列分析 修伟明，董双林，苗 慧，穆兰芳 （南京农业大学/农业部病虫监测与治理重点开放实验室，南京 210095 ） 摘要: 通过比较几种已发表夜蛾科昆虫的信息素结合蛋白（PBP）氨基酸序列，设计合成一对简并性引物, 利 用反转录多聚酶链式反应（RT-PCR）技术从甜菜夜蛾(Spodoptera exigua Hübner)雄虫触角扩增得到2个分别为 275 bp 和 281 bp 的 cDNA 片段 SexigPBP1 和 SexigPBP2，其分别由 92 个氨基酸残基和 94 个氨基酸残基组成。通 过在GenBank 中进行序列的同源性比较，表明这2个序列与已知几种昆虫的PBP氨基酸序列具较高同源性。 关键词：甜菜夜蛾；信息素结合蛋白基因；序列分析 Molecular Cloning and Sequence Analysis of Two cDNA Fragments of Pheromone Binding Protein in Male Beet Armyworm, Spodoptera exigua Hübner XIU Wei-ming, DONG Shuang-lin, MIAO Hui, MU Lan-fang (Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095) Abstract: By reverse transcription-polymerase chain reaction (RT-PCR) method with a pair of degenerate primers, two cDNA fragments of pheromone binding protein （PBP ）gene, SexigPBP1 and SexigPBP2, were cloned and sequenced from the antennae of male Spodoptera exigua. SexigPBP1 and SexigPBP2 contained 275 bp and 281 bp, and with deduced amino acid sequences of 92 residues and 94 residues, respectively. The sequence analysis indicated that the deduced amino acid sequences of the cDNA fragments shared high identity with PBPs gene sequences from other previously reported insects. Key words: Spodoptera exigua; Pheromone binding protein gene; Sequence analysis 甜菜夜蛾(Spodoptera exigua Hübner)属鳞翅目夜 于开发有效的行为调控技术来防治害虫和利用益虫， 蛾科，是一种世界性重要害虫。在中国南方已成为常 也具有重要价值。Vogt 和 Riddiford 用标记性信息素 发性害虫，在北方亦成为严重的害虫，特别是近年来 的方法，首次在多音大蚕蛾Antheraea polyphemus 雄 在棉花上的为害，更应引起高度重视[1] 。由于频繁使 蛾触角中发现昆虫 PBP [3] ，迄今为止已从近