脑缺血再灌流时脑温的动态改变.pdfVIP

· 4 · 生堡膻 簧痘 志 (鱼壬 ) 生 月第3卷第3期ChinJ 6 Ds／(E 泐n fn)，June2009．vo1．3．No．3 · 基础研究 · 脑缺血再灌流时脑温的动态改变 张洪 章军建 梅元武 孙圣刚 童萼塘 【摘要】目的 了解脑缺血再灌流后脑温的变化，为脑缺血动物的实验研究、以及亚低温脑保护 作用机制提供理论依据。方法 将 SD大鼠分为四动脉阻断 、双颈总动脉阻断 、单侧颈总动脉阻断以 及四动脉阻断 10min、20min、30min后再灌流组。用点式测温仪同时测量不同部位脑组织的温度。 结果 脑组织内存在温度梯度；脑缺血后同一时间点纹状体、海马和颞肌的温度不一致；不同程度 脑缺血后脑温的变化不一致，双侧颈总动脉阻断的脑缺血中脑温的改变呈波浪状，单侧颈总动脉阻断 的脑缺血中缺血侧脑温低于对照侧 ；四动脉阻断的全脑缺血中，缺血后不同时间开始再灌流脑温都有 回升现象；脑缺血的严重程度和持续时间可影响动物的清醒和脑温的变化。结论 本实验的结果说 明了在脑缺血实验研究时采用连续监测、并将脑温控制在特定的范围内，避免脑缺血和再灌流期间脑 温的波动影响实验结果的准确性。 I关键词】脑缺血；再灌流；脑温；大鼠 Dynamicchangesofbrain temperature in ratduring cerebralischemia andreperfusion ZHANG ng，ZHANGJun-jian，MEIYuan—wu，SUN Sheng—gang，TONGE—tang．DepartmentofNeurology， ZhongnanHospitalofWuhanUniversity；CenterforCerebralVascularDiseases，MedicalCollegeofWuhan University，Wuhan430071，China Correspondingauthor：ZHANG Hong，Email：zhangh9@ yahoo．corn 【Abstract】Objective Inordertostudytheneuroproteetiveeffectofmildhypothermiaoncerebral icshemia，braintemperatureduringcerebralischemiaandreperfusionwereinvestigated．Methods Spra— gue—Dawley(SD)ratsweredividedinto6groups：4-vesselsocclusion，bilateralcommoncarotidarteries (CCA)occlusion，lateralCCAocclusion，reperfusionfollowing4一vesselsocclusionatlOmin，20minnad 30min intervals．Brain temperature were continuously simultaneously recorded at different brain re— gion~Results Temperaturegradientexistsinbraintissue．Braintemperaturergaduallydecreasesduring cerebral ischemi ＆Braintemperatureinischemiehemisphereislowerthna thatinnon—ischemieoneas cere- bral ischemiaisinducde bylateral CCA occlusion．Braintemperatureelevatde trnasientlyat theeralystageof reperfusion，thendecreased，andrgaduallyreturnedtobaselineas timeofnaesthesiapassed．111eseverity and duration of cerebral isehemia Callaffectna imalto regain consciousness and its brain tempera- ture．Condusions Inordertogettheaccurat