渝荣Ⅰ号猪配套系品系间DNA甲基化水平的差异研究.pdfVIP

：一实验设计、匀 扛尺性状的聚类： rendonkJAM． aaponentsregres 渝荣 Ⅰ号猪配套系品系间DNA甲基化水平的差异研究 白小青1 王金勇1 陈英2 潘红梅2 刘 文 1 李军峰1,3 1.重庆市畜牧科学院 重庆荣昌 4024602.西南大学动物科技学院 重庆北碚400715 3.重厌市科学技术信息中心 重庆渝北401147 摘要 应用HPLC方法检测了90头30日龄渝荣 Ⅰ号猪配套系A系、C系、CB系猪耳 组织基因组DNA的甲基化程度，探讨品系间DNA甲基化水平的差异。结果显示，A系、C 系、CB系猪仔猪耳基因组DNA甲基化水平分别为2.4916％、2.5971％和1.0598％。以C 系最高，A系次之，且两者间差异不显著；CB系最低，且极显著低于A系和C系。研究结果 为探索商品代杂种优势的分子机制提供理论依据。 关键词 渝荣 Ⅰ号;高效液相色谱;DNA甲基化 TheStudyonStrainSpecificDifferences inLevelsofDNAMethylationofYURONGIPig Mutiple-linesCrossSystems Bai-xiaoqing1Wang-jinyong1 Chen-ying2 Pan-hongmei1Liu-wen1 Li-junfeng1,3 1.ChongqingAcademyofAnimalSciences,Rongchang, Chongqing402460,China;2.AnimalScienceTechnologyCollegeofSouthwest University,Beibei,Chongqing400715,China;3.ScienceandTechnology InformationCenterofChongqing,Yube,Chongqing401147,China Abstract:Byhighperformanceliquildchromatography(HPLC),90YURONG Ⅰpigmuti pie-linescrosssystemspigsattheageof30d,suchasA,BandCBstrainpigsweredetectedfor thetotalgenomicDNAmethylationlevelsofears.ThestrainspecificdifferencesinlevelsofDNA methylationwerealsoanalyzed.TheresultshowedthatthegenomicDNAmethylationcontentsof A,CandCBstrainpigswereseparately2.4916％、 2.5971％ and1.0598％.DNAmethyla tionlevelsofCstrainpigswerethehightestofthreestrains,buttherewerenotsignificantdiffer encebetweenClinepigsandAlinepigs(P＞0.05).ThatofAstrainpigswerehigherthanCB linepigsandsignificantdifferencehappenedbetweenCBlinepigsandAlinepigs(P＜0.01),al sobetweenCBlinepigsandClinepigs(P＜0.01).Theresltprovidednecessarydatasforpro bingintothemolecularmechanismsofheterosisincommercialpigs. Keywords:Yurong ⅠPig;HPLC;DNAmethylation II0,00110,012,001 -甲基脱氧胞苷 PeskB.13.83rain ：标