青枯雷尔氏植物疫苗菌60 Co 诱变菌株生物学特性.docVIP

青枯雷尔氏植物疫苗菌60Co诱变菌株的生物学特性 周游1,2，郑雪芳2，刘波2(，黄建忠1，车建美2 （1.福建师范大学生命科学学院，福建 福州 350008；2.福建省农业科学院农业生物资源研究所，福建 福州 350003） 摘 要：利用60Co辐射诱变青枯雷尔氏菌无致病力菌株FJAT-1458构建其突变体库，比较诱变后菌株与出发菌株在生长特性、防效、定殖方面的异质性。结果表明，60Co 诱变处理菌株的最佳剂量为200Gy，致死率达到92.5%。52株60Co辐射诱变菌株中，有7株菌能提高对番茄青枯病的防治效果，为正突变，占13.5%有11株菌的生防效果明显低于出发菌株的防治效果，负诱变率占21.6%，其中60Co 诱变后菌株FJAT-15022对番茄青枯病生防效果最好，在接种致病性青枯雷尔氏菌后第15d，对番茄青枯病防治效果达91.7%，明显高于出发菌株（83.3%）。60Co 诱变后菌株FJAT-15022的生长特性与出发菌株FJAT-1458不同，菌株FJAT-15022能在较高温度(40℃)正常生长，而出发菌株FJAT-1458在此温度下不能生长；此外，二者的生长曲线不同，主要表现在对数生长期，在此期间，菌株FJAT-15022生长速率大于出发菌株FJAT-145860Co 诱变后菌株FJAT-15022的定殖特性也与出发菌株FJAT-1458不同，菌株FJAT-15022的定殖时间比出发菌株FJAT-1458延迟5d，但出发菌株FJAT-1458的定殖数量高于诱变后菌株FJAT-15022。本研究为研制防治青枯病的植物疫苗菌剂提供了基础资料。 关键字：青枯雷尔氏菌；诱变；温度；生物防效；定殖 iological characteristics of radioactive mutagenic of Ralstonia solanacearum by 60Co ray as a plant vaccine Zhou You1,2, Zheng Xuefang2, Liu Bo2*, Huang Jianzhong1, Che Jianmei2 （1College of Life Science Fujian normal university, Fuzhou 350108, China; 2Biotechnology Institution, Fujian Academy of Agricultural Sciences, Fuzhou 350003） Abstract:iological characteristics of radioactive mutagenic of Ralstonia solanacearum by 60Co ray as a plant vaccine. The avirulent strain of Ralstonia solanacearum FJAT-1458 was treated by 60Co ray to set up the mutant library, from which the characteristics of bio-control efficiency on the bacterial wilt disease and colonization on the host were compared between the mutants and the original strain. The results showed that the optimum dose of 60Co mutagenic treatment was 200Gy. 92.5% of the strains treated to form the mutants, of which 13.5% displayed the positive action on raising bio-control efficiency of R. solanacearum, on the other hand, 21.6% revealed the negative action on decreasing bio-control efficiency, comparing to the original strain, respectively. The 15-day bio-control efficiency of the mutant FJAT-15022 on the bacterial wilt disease was the best with 91.7%, significantly higher than original strain FJAT-1458 (83.3%).