复方参芪五味咀嚼片水提取工艺的研究.pdfVIP

研究论文 复方参芪五味咀嚼片水提取工艺的研究 史国兵1 赵明宏1 荆、学惠1 何静1 李文韬1’2 (1．沈阳军区总医院药剂科沈阳110840；2．辽宁医学院) 摘要目的：研究复方参芪五味咀嚼片最佳水提取工艺。方法：以黄芪甲苷含量为指标，结合出膏率，进行L9(34)正交试 mm×4．6 验，筛选最佳工艺条件，采用HPLC—ELSD法测定黄芪甲苷的含量。色谱条件：色谱柱为DikmaDiamonsil—C。。(250 mm，5 Ixm)，流动相为乙腈一水(32：68)，蒸发光散射检测器检测．流速为1．0ml·min～，柱温为室温。结果：最佳提取条件为10 倍量水煎煮提取3次，每次1．5h。结论：本工艺提取完全，且检测方法简单，专属性强，重复性好。 关键词 复方参芪五味咀嚼片；黄芪甲苷；提取工艺 中图分类号：R284．2文献标识码：A 文章编号：1008-049X(2011)06-0762-03 onWaterExtraction Processof WuweiChewableTableIs Study CompoundShenqi Shi Xuehui of of Mili— Guobin91，Zhao Minghon91，Sun1，HeJin91，LiWenta01’2(1．DepartmentPharmacy，GeneralHospitalShenyang 1 Medical taryDistrict，Shenyang10840，China：2．LiaoningUniversity) ABSTRACT establishthe extractionof Wuweichewable or— Objective：To optimal processcompoundShenqi table to theextractionof Wuweichewabletabletsandthecontent thogonaldesignk(34)wasapplieddesign processcompoundShenqi of IVwasdeterminedHPLC—ELSD．Thecontentof IVandtheextractionofcrude wereusedas astragaloside by astragaloside yield drugs theindicatorsto theextraction mm×4．6 wasused．Themobile Diamonsil．C，女(250mm，5LLm)column optimize technology．Dikma consistedofacetonitrile—water(32：68)withaflowrateof1．0m卜min～atroom extraction phase temperature．Result：Theoptimal wasas extractionfor3timeswith10一foldvolumeofwaterand1．5hourseach extrac— process follows：decocting time．Conclusion：The tioniS the entire，anddetectionmethodsare and simple，sensitivereproducible． KEYWOR