微波法提取蜈蚣藻多糖工艺及抗氧化活性的研究【毕业论文】.doc

本科毕业论文 （20 届） 微波法提取蜈蚣藻多糖工艺及抗氧化活性的研究 专业：药学 目录 摘 要…………………………………………………………………………………1 ABSTRACT ……………………………………………………………………………2 1 前言 …………………………………………………………………………………3 2实验材料、试剂与器材 ……………………………………………………………4 3实验方法………………………………………………………………………………4 3.1蜈蚣藻多糖的提取………………………………………………………………4 3.2蜈蚣藻多糖抗氧化活性测定 …………………………………………………5 4实验结果 ……………………………………………………………………………6 4.1葡萄糖标准曲线制作……………………………………………………………6 4.2单因素实验………………………………………………………………………6 4.3正交实验…………………………………………………………………………8 4.4多糖抗氧化研究…………………………………………………………………9 5讨论 …………………………………………………………………………………9 6结论 …………………………………………………………………………………10 参考文献 ……………………………………………………………………………致谢 …………………………………………………………………………………附录Ⅰ文……………………………………………………………………13 附录Ⅱ文文 ……………………………………………………………………[摘要]利用DPPH法、清除羟自由基法和清除自由基的能力法进行抗氧化实验。结果[关键词] ；抗氧化； DPPH ；羟自由基Study on Microwave-assisted extraction and the antioxidant activity of water-soluble polysaccharide from Grateloupia filicna [Abstract] Objective:extract water-soluble polysaccharide by Microwave-assisted extraction and determine its antioxidant activity.Methods: Methods：Microwave-assisted extraction;The content of polysaccharide was determined by phenol-sulphuric acid methed;The anti-oxidation was measured by DPPH method and free radical scavenging action of ·OH ( the self oxidation method of pyrogallol Fenton system) Results:Under the optimum parameters of microwave power of 600W,extraction temperature of 90℃,extraction time of 10min and liquid-to-solid ratio of 70,the yield of crude polysaccharides reached to 18.13%.The microwave power is the biggest effect factors.Conclusion: Grateloupia filicna polysaccharide has the effects of　DPPH and ·OH. scavenging the elimination rate of the production have some connection with the concentration of polysaccharide. [Key words] Grateloupia filicna,Antioxidant,DPPH,Hydroxyl radical,Superoxide radical 1 前言 1.1蜈蚣藻和多糖 蜈蚣藻(Grateloupia filicina C. Ag.)，别名海赤菜、冬家烂、膏菜。属于红藻门、真红藻纲、隐丝藻目、海膜科、蜈蚣藻属[1]。藻体呈紫红色，胶质粘滑，丛生，主干单一至顶，亚圆柱形略扁，不规则地羽状分枝1-3次，互生、对生或偏生。体表有时可见颗粒状囊果[2]。 多糖具有广泛的的生物活性与功能, 如降血糖[3