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大七气汤对大鼠子宫内膜异位症P450与COX-2表达的影响 张沁舒，方毅，王力，陈蓉 (400016 重庆,重庆医科大学中医药学院) ［摘要］ 目的 探讨大七气汤加减方（MDQD）治疗内异症的机理。方法 采用SD雌性大鼠子宫内膜自体移植法建模, 建模3周后，随机分为大七气汤加减方高剂量组（A组，n=9）与低剂量组（B组，n=9）、孕三烯酮组(C组,n=9)、阳性对照组（D组,n=10）及假手术组（E组,n=10）。给药3周后,用发光免疫分析法和ELISA法检测大鼠血清与异位内膜组织雌二醇（E2）与前列腺素E2（PGE2），用免疫组化法和RT-PCR技术检测异位组织芳香化酶P450(P450arom)、环氧化酶2(COX-2)蛋白与mRNA的表达。结果 MDQD高剂量组大鼠血清E2[(25.38±2.28)pg/ml]、PGE2[(27.17±4.67)ng/L]，组织E2[(1.07±0.10)ng/g]、PGE2[(20.73±3.78)ng/L]，P450蛋白(22.58±4.84)及mRNA（0.377±0.039），COX-2蛋白（4.70±0.73）及mRNA（0.144±0.011）明显低于模型组（P0.01）,与孕三烯酮组比较无明显差异（P0.05）。结论 大七气汤加减方能降低内异症大鼠P450、COX-2和PGE2的含量，通过抑制雌激素生成代谢降低外周血与局部病灶雌激素水平，抑制异位内膜生长。 ［关键词］ 大七气汤；子宫内膜异位症；芳香化酶P450；环氧化酶-2；雌激素 ［中图法分类号］ R271.1； R289.5 ［文献标志码］ A Effect of Da Qiqi Decoction on the expression of P450 and COX-2 in endometriosis of rats Zhang Qinshu，F Yi，W Li，C Rong （School of Traditional Chinese Medicine，Chongqing Medical University，Chongqing，400016，China） ［Abstract］ Objective To explore the therapeutic mechanism of Modifided Da Qiqi Decoction（MDQD）for endometriosis.Methods The endometriosis model was established in female SD rats.After 3 weeks，the were randomly divided into high dose group of MDQD (group A, n=9)，low dose group of MDQD (group B, n=9)，gestrinone group(group C,n=9)，positive control group (group D,n=10)and Sham-operation control group(group E,n=10).After 3 weeks of the treatment, the levels of E2 and PGE2 in serum and ectopic endometrial tissues were measured by ECLIA and ELISA.The expression of protein and mRNA of P450 and COX-2 in ectopic endometria were determined by immunohistochemistry and RT-PCR.Results Compared with positive control group，high dose group of MDQD declined significantly in the serum levels of E2[(25.38±2.28)pg/ml] and PGE2[(27.17±4.67)ng/L]，the tissues contents of E2 [(1.07±0.10)ng/g] and PGE2[(20.73±3.78)ng/L]，the expression of P450 protein(22.58±4.84) and mRNA（0.377±0.039），COX-2 protein（4.70±0.73） and mRNA（0.144±0.011）(P0.01). There was no significant difference between high dose group of MDQ