脂质体介导含RGD序列内皮抑素基因抑制角膜新生血管的研究.pdfVIP

眼科研究2010年 1月第 28卷第 1期 · 19 · 实 验 研 究 · 脂质体介导含 RGD序列内皮抑素基因抑制角膜 新生血管的研究 刘 平 李 华 张 红 张丽娟 宋 甄 葛红岩 Inhibition ofcornealneovascularization by liposomemediatedplasmid encoding endostatin withRGD sequence LiuPing，LiHua，ZhangHong，ZhangLijuan，SongZhen，GeHongyan．DepartmentofOphthalmology， AffiliatedFirstHospitalofHarbinMedicalUniversity，Harbin150001，China Abstract Background IthasbeendemonstratedthatⅨp3／ p5andⅡ513lintegrinsareoverexpressedinneovascular tissue．Consequently，peptidecontainingtheRGD (Arg—Gly—Asp)sequence，whichexistsinligandsofintegrins，iseffectivein targetingtherapeuticreagentstoneovascularendothelium． Objective Presentstudyaimstoinvestigatetheantiangiogenetive effectsofliposomemediatedplasmid encoding endostatinwith RGD sequenceon alkaliburn—inducedcornea1neovascularization (CNV)inrabbits． Methods CNVmodelsinducedbyalkaliburnwereestablishedin72eyesof36NewZealandwhiterabbits byputtingthefilterpaperwith 1inol／L NaOH atthecentralcorneafor20 seconds．Theanimalmodelsweredivided intofour groupsrandomly．0．2mL ofliposomeandplasmid encodingRGD—ES complex (1iposomemediatedpCI—RGD—ES injection group)，liposomeandplasmidencodingEScomplex (pCI—ESinjectiongroup)，liposomeandcarrierplasmid (pCI)complex (pC1．ESinjectiongroup)，andPBSweresubconjunctivallyinjectedrespectivelyinthemodelsfrom fourgroupstwiceaweekofr twoweeks．Thegrowth statusofCNV wasobserved off day 1，3，7，14 after alkaliburn under the slim lamp microscope． Experimentalanimalswere sacrificed on the3rd，7th and 14th day and the expression ofVEGF in CNV wasdetected by immunohistochemistry．ThenumberofcornealmicrovesselswascountedbasedonthenumberofCNV cross—sectionunderthelight microscope． Results CNV areawassignificantlysmallerinliposomemediatedpCI—RGD—ESinjectiongroupandpCI—ES