永生化肝细胞移植治疗肝衰竭_医学论文.docVIP

永生化肝细胞移植治疗肝衰竭_医学论文 永生化肝细胞移植治疗肝衰竭_医学论文 作者：李羽，王军，徐哲，张岩，白雪帆 【关键词】 永生化 　　Immortalized hepatocyte transplantation for treating liver failure　　【Abstract】 AIM: To study the roles of immortalized and primary human fetal hepatocyte intrasplenical transplantations in treating the rats with acute liver failure (ALF) induced by Dgalactosamine (Dgal). METHODS: ALF rats were induced by Dgal, and were divided into 3 groups. Rats of groupⅠreceived 2×107 immortalized human fetal hepatocytes intrasplenically rats of group Ⅱreceived 2×107 primary human fetal hepatocytes intrasplenically rats of group Ⅲ received 1 mL normal saline intrasplenically. All rats received Cyclosporin A(CsA) at 10 mg/kg im simultaneously. Then the survival rates, liver function and liver histology of all rats were observed. RESULTS: Survival rates of rats in groupⅠand Ⅱ were obviously higher than that of group Ⅲ (72.2% vs 22.2%, 61.1% vs 22.2%, 0.01) the liver function and liver histology were also improved significantly as compared with those of group Ⅲ. Survival rate of group Ⅰ was close to that of groupⅡ (72.2% vs 61.1%, Pgt0.05). CONCLUSION: After human fetal hepatocyte transplantation intrasplenically, the survival rate of ALF rats intoxicated with Dgal is increased, and the liver function and histology are also improved. The immortalized hepatocytes would become the ideal cell material in the study of hepatocyte transplantation.　　【Keywords】 immortalization fetal hepatocytes transplantation liver failure, acute Dgalactosamine 　　【摘要】 目的： 观察永生化胎肝细胞及胚胎肝细胞经脾脏移植对D氨基半乳糖（Dgal）诱导的急性肝衰竭大鼠的治疗作用. 方法： 采用Dgal诱导大鼠急性肝衰竭，分3组进行治疗实验，Ⅰ组： 经脾脏移植永生化胎肝细胞2×107个；Ⅱ组： 移植原代胚胎肝细胞2×107个；Ⅲ组： 脾脏注射生理盐水1 mL，各组大鼠同时肌肉注射环孢霉素A(CsA) 10 mg/kg. 观察大鼠的存活率、肝脏功能、肝脏病理变化情况. 结果： Ⅰ组及Ⅱ组大鼠存活率均显著高于Ⅲ组大鼠（72.2% vs 22.2%, 61.1% vs 22.2%, P＜0.01），肝脏功能及肝脏病理均有改善, Ⅰ组与Ⅱ组之间大鼠存活率无明显差异(72.2% vs 61.1%, P＞0.05). 结论: 经脾脏移植胚胎肝细胞可明显改善Dgal诱导的肝衰竭大鼠的存活率，改善肝功能及肝脏病理，该永生化胎肝细胞可以成为肝细胞移植研究中的理想细胞材料.　　【关键词】 永生化；胚胎肝细胞；移植；肝功能衰竭，急性；D氨基半乳糖 　　0引言 　　肝功能衰竭是各种肝脏疾病患者死亡的主要原因. 目前原位肝脏移植是挽救肝衰