DNA and RNA isolation and purification (course readings .pptVIP

DNA and RNA isolation and purification (course readings 10 and 11) Genomic DNA preparation overview Plasmid DNA preparation DNA purification Phenol extraction Ethanol precipitation RNA work Bacterial genomic DNA prep: cell extract Genomic DNA prep: removing proteins and RNA 2 ways to concentrate the genomic DNA Genomic DNA prep in plants -- how get rid of carbohydrates? Plasmid purification: alkaline lysis DNA purification: silica binding In vitro amplification of DNA by PCR Theory of PCR Components of the PCR reaction A few advanced applications of PCR Reverse transcription PCR (for R