血浆组分Ⅳ中载脂蛋白A-1纯化工艺研究.pdfVIP

·30· 血浆组分Ⅳ中载脂蛋白A—I纯化工艺的研究 黄凯何秋包骧飞 李军辉△ 李春洲 郭颀然 朱金许必雄 (上海莱士血液制品股份有限公司，上海200245) 摘要：目的开发从血浆组分IV中制备载脂蛋白A-I(apolipoproteinA·I，apoA．I)的纯化工艺，获得高纯度 apoA-I蛋白。方法组分Ⅳ沉淀经复溶、过滤预处理后，滤液经两步离子交换层析，获得apoA．I纯品。以免疫沉 淀法鉴定目的蛋白，并以SDS电泳检测纯度。结果 经离子交换纯化后获得了高纯apoA．I蛋白，蛋白纯度 达95％，蛋白回收率58．8％。结论此工艺能够从组分IV中纯化得到高纯度apoA·I蛋白，纯化过程具有很好的 稳定性，纯化工艺易干放大，适于大规模生产。 关键词：血浆组分IV；高密度脂蛋白(HDL)；载脂蛋白A—I Purificationof Plasma ApolipoproteinA·I(apoA-I)fromFractionⅣKieuHo，Bao Hoang，Thu XianKfei，Li RAilSBloodProducts Junhui，LiChunzhou，GuoQiran，Zhu^nquan，ShueChris．Shanghai 200245， Company，Shanghai ， China To a for Abstract：0bjective developpurificationtechnologypreparinghi#ypurifiedApolipoproteinA-I(apoA· from fractionIV．MethodsPlasma isredissolvedandfiltrated．Thefiltrateis I)proteinplasma fractionⅣprecipitate two ofion isidentified andthe purifiedby steps exchangechromatography．ThepurifiedapoA·I byimmunoprecipitation isdetectedSDS·PACE with isobtainedafterthesecondehrom；t- purity by eleetrophoresis．R姻IIltsApoA-1hishpurity total was to95％．Conclutioncanbe this yield tographystep．The 58．5％．ApoA-1w鹊purified ApoA—I purifiedby with resolusion．ThisCanbe scaledfor scale technologyhigh technologyeasily up large production． fraction Keywords：plasmaIV；High A-I DensityLipoprotein；Apolipoprotein 高密度脂蛋白(HDL)可将末梢组织的游离胆设备厂。层