ERK信号通路在小鼠变应性鼻炎发病机制中及研究.pdfVIP

华 中 科 技 大 学 硕 士 学 位 论 文 中 文 摘 要 目的：观察细胞外信号调节激酶（external signal regulated kinase, ERK ）的磷酸化 程度在变应性鼻炎（allergic rhinitis ）小鼠模型中的表达，以探讨ERK 信号通路转导 途径在变应性鼻炎中的作用机制。 方法：成年小鼠20 只随机分成变应性鼻炎组和对照组，每组10 只，以腹腔内注 射联合鼻腔滴注卵清蛋白（OVA ）复制变应性鼻炎小鼠模型，以组织病理染色，免疫 组织化学和图像分析方法测定小鼠鼻中隔粘膜磷酸化 ERK （Phospho-ERK, P-ERK ） 表达及鼻腔黏膜形态学改变。 结果：变应性鼻炎组中鼻中隔黏膜层纤毛破坏程度，杯状细胞数，嗜酸性粒细胞 数均较正常组增高，免疫组化显示鼻粘膜组织中p-ERK 表达变应性鼻炎组显著高于对 照组（p 0.05 ）。 结论：ERK 磷酸化水平在变应性鼻炎小鼠鼻黏膜中的表达均增加，提示ERK 信 号转导途径在变应性鼻炎的发生机制中起相关作用。 关键词：变应性鼻炎；细胞外信号调节激酶 2 华 中 科 技 大 学 硕 士 学 位 论 文 ABSTRACT Emchanisms of ERK in mouse models of allergic rhinitis Objective: To elucidate the emchanisms of external signal regulated kinase(ERK) in murine allergic rhinitis models. Method: 20 Grown-up mice were randomly divided into two groups, The allergic rhinitis (AR)group and the control group. Every group has 10 mice. An AR mouse model was developed by means of ovalbumin(OVA) intraperitioneal injection and subsequent repeated OVA intranasal chakkenge. AR models were examined by hematoxylin and eosin stain, periodic acid-Schiff stain, general morphology immunohistochemistry methods, the number of p-ERK cells in epithelium was also counted. Results: In AR model group, the destory of mucous membrane in anasl septum, acidophilic granulocyte, goblet cells were significantly increased, compared with the control group. Immunohistochemistry shows that the expression of p-ERK in anasl septum is significantly increased, compared with the control group(p 0.05). Conclution: The expression of p-ERK was increased in allergic rhinitis models, so erk might paly a crucial role in the emchanisms of allergic rhinitis. Keyword: Allergic rhinitis; ERK