虎杖苷对烧伤血清和LPS作用下大鼠平滑肌细胞蛋白激酶C活性的影响_医学论文.docVIP

虎杖苷对烧伤血清和LPS作用下大鼠平滑肌细胞蛋白激酶C活性的影响_医学论文 虎杖苷对烧伤血清和LPS作用下大鼠平滑肌细胞蛋白激酶C活性的影响_医学论文 作者：王月刚，金春华，黄海潇，吴平生 【关键词】 虎杖苷 基金项目：国家自然科学基金 【Abstract】 AIM: To explore the mechanism of polydatin (PD) treating shock through studying the influence of PD on the activity of protein kinase C (PKC) of vascular smooth muscle cells (VSMC) when treated by sera from burned rats and lipopolysaccharide (LPS). METHODS: After VSMC was treated by sera from burned rats or LPS, the activity of PKC was measured by γscintillation counting instrument. RESULTS: Activity of PKC of VSMC cytoplasm after treated by sera from burned rats decreased (0.05 vs normal group), but activity of PKC of cell membrane increased (0.05 vs normal group). When VSMC was treated with PD, the activity of PKC of VSMC cytoplasm increased and that of cell membrane decreased (0.05 vs burned group and control group, Pgt0.05 vs normal group). However, the activity of PKC of VSMC cytoplasm and cell membrane treated by LPS increased (0.05 vs normal group), after PD treated VSMC the activity of PKC decreased again (0.05 vs LPS group and control group, Pgt0.05 vs normal group). CONCLUSION: PD antagonizes the damage effects of LPS and sera of burned rats by reversing the PKC activities, which may be one of the molecular mechanisms of PD antishock property. 【Keywords】 burn lipopolysaccharide smooth muscle cell polydatin protein kinase C 【摘要】 目的:观察虎杖苷(PD)对烧伤血清和脂多糖（LPS）作用下大鼠血管平滑肌细胞（VSMC）蛋白激酶C（PKC）活性的影响，探讨其抗休克的机制. 方法:取大鼠胸主动脉培养VSMC，用烧伤血清和LPS刺激VSMC后，γ闪烁计数仪测定PKC活性. 结果： 烧伤血清作用后VSMC胞质PKC的活性下降(vs正常组0.05)，但胞膜的PKC活性上升（vs正常组0.05），经PD治疗后胞质PKC的活性上升（vs烧伤组、治疗对照组0.05，vs正常组Pgt0.05），胞膜的PKC活性下降（vs烧伤组、治疗对照组0.05, vs正常组Pgt0.05）. LPS刺激后VSMC胞质与胞膜PKC的活性均上升（vs正常组0.05），经PD处理后其活性又均下降（vs LPS组、治疗对照组0.05，vs正常组Pgt0.05）. 结论： PD对烧伤血清和LPS作用下的VSMC的保护作用可能是通过调节PKC的活性来发挥作用的，这可能是其抗休克的分子机制之一. 【关键词】 烧伤；脂多糖；平滑肌细胞；虎杖苷；蛋白激酶C 0引言 虎杖苷(polydatin, PD)是从中药虎杖中提取的主要成份，在体动物实验表明PD对烧伤性、感染性休克有良好疗效，治疗休克动物的存活时间和存活率均明显优于6542和多巴胺［1］. 平滑肌细胞(smooth muscle cell, SMC)在小血管的收缩扩张中占据着主导地位，而蛋白激酶C（protein