食管鳞癌中zac基因的表达及临床意义_医学论文.docVIP

食管鳞癌中zac基因的表达及临床意义_医学论文 食管鳞癌中zac基因的表达及临床意义_医学论文 作者：祁秋干，马军，达嘎，闫尧，唐芙爱，李印 【摘要】 　　目的：观察zac基因在食管癌中的表达及意义. 方法：采用RT检测36例食管鳞状细胞癌组织，相应的癌旁组织和远癌组织中zac mRNA的表达情况. PCR产物经凝胶电泳，比较3种组织中zac与GAPDH条带的灰度值之比，半定量分析zac mRNA的表达水平. 结果：36例食管癌组织中有14例(38.9%) zac mRNA检测到阳性表达，22例(61.1%) zac mRNA表达缺失，其阳性表达低于相应的癌旁组织（85.5%）和远癌组织(100.0%, 0.05)；食管癌组织中zac mRNA阳性表达水平(0.38±0.13)低于相应的癌旁组织(0.72±0.16)和远癌组织(0.80±0.12)；随着肿瘤分化程度的升高，zac mRNA在食管癌组织中的阳性表达率也增加(0.05)；zac mRNA阳性表达率在无淋巴结转移的癌组织中显著高于有淋巴结转移者(0.05)，而临床病理分期和病理类型比较差异无统计学意义(Pgt0.05). 结论: zac mRNA在食管癌组织中存在表达缺失和低表达，说明zac基因在食管癌的发生发展中可能具有抑癌基因作用，可能与食管癌的转移密切相关. 【关键词】 zac基因；食管肿瘤；mRNA；逆转录多聚酶链反应 　　【Abstract】 AIM: To study the expression of zac gene in esophageal squamous cell carcinoma and its significance. METHODS: The expression of zac gene was detected by RTPCR in 36 cases of esophageal cancer tissues, matched paracancer tissues and tissues far away from tumor. PCR products were tested by gel electrophoresis. The expressions of zac gene in the three kinds of tissues were semiquantified by calculating the ratio of zac and GAPDH gray value of bands respectively. RESULTS: The positive expression rate of zac mRNA in 36 cases of esophageal carcinoma was 38.8% (14/36), the negative expression rate of zac mRNA was 61.1% (22/36). The positive rate was lower than that in tumoradjacent tissues (80.5%) and esophageal mucosa far away from tumor (100.0%), respectively (0.05). Semiquantified analysis showed that the expression level of zac mRNA in esophageal cancer tissues (0.38±0.13) was lower than that in tumoradjacent tissues (0.72±0.16) and esophageal mucosa far away from tumor (0.80±0.12). The higher positive expression rate of zac mRNA was correlated with higher differentiation grades in cancerous tissues (0.05). The positive rate of zac mRNA expression was higher in cancerous tissues without lymph node metastasis than in cancerous tissues with lymph node metastasis (0.05), but it was not correlated with TNM stages and histologic types of tumor (Pgt0.05). CONCL