动脉粥样硬化兔模型中心肌细胞凋亡的观察_临床医学论文.docVIP

动脉粥样硬化兔模型中心肌细胞凋亡的观察_临床医学论文 动脉粥样硬化兔模型中心肌细胞凋亡的观察_临床医学论文 作者：李明哲 卜培莉 陈文强 孙敬春 【关键词】 心肌细胞凋亡 　　【摘要】 目的 观察兔动脉粥样硬化（AS）模型中心肌细胞的凋亡现象。 方法 采用高胆固醇饮食及腹主动脉球囊拉伤的实验方法，建立兔AS模型，12周行腹主动脉血管超声检查后，处死动物，制作切片，行透射电镜观察。 结果 新西兰白兔共29只，存活24只，存活率83%。血管超声示高胆固醇组及高胆固醇加腹主动脉球囊拉伤组（以下简称干预组）腹主动脉内均可见斑块影;对照组则未见斑块影。各组兔体重差异无显著性（Pgt;0.05）。干预组TC、TG、LDL均值明显高于对照组及实验前水平，0.001;而对照组实验前后TC、TG、LDL等指标差异无显著性（Pgt;0.05）。电镜下对照组结构正常;两干预组心肌组织标本则呈现心肌细胞凋亡及心肌纤维组织增生的改变。 结论 新西兰白兔AS模型中，AS早期即尚未出现心功能障碍时，心肌细胞即出现凋亡的增多，并伴有心肌纤维化的出现，心肌细胞凋亡不仅是产生心功能减退的重要机制，心肌细胞凋亡本身很可能是心肌纤维化的诱因。 　　关键词 动脉粥样硬化 心肌细胞凋亡 模型 　　Observation of cardiomyocyte apoptosis in atherosclerotic model of rabbit 【Abstract】 Objective To explore cardiomyocyte apoptosis in physiological and pathological of atherosclero-sis.Methods A atherosclerotic model of rabbit was established by feeding hypercholesterol diet for12weeks and bal-lon injuring on ventroaorta.After12weeks，all hypercholesterolaemic rabbits were measured by blood vessel ultrasonic in older to examine atherosclerotic blocks.The left ventricular tissue was used for the observation of cell with the method of electron microscope.Results The animal liavbility was83%（24/29）in the course of researching.As a result，normal group，hypercholesterol diet group，ballon injuring on ventroaorta with feeding hypercholesterol diet group.The two latter groups were examined by blood vessel ultrasonic and showed blocks on ventroaorta.At the end of research the weight of these groups had no significant differences.The statistical figure indicate that TC，TG，LDL of hypercholesterol diet group and ballon injuring on ventroaorta with feeding hypercholesterol diet group had no signifi-cant differences with control group in the beginning of research（Pgt;0.05）.But after research the levels of TC，TG，LDL were significant higher（0.001）in hypercholesterol diet group and ballon injuring on ventroaorta with feed-ing hypercholesterol diet group than before.The control group was contrary（Pgt;0.05）.Under the electronic light microscope the control gr