坐骨神经周围注射可乐定对CCI大鼠促炎性细胞因子表达的影响_临床医学论文.docVIP

坐骨神经周围注射可乐定对CCI大鼠促炎性细胞因子表达的影响_临床医学论文 坐骨神经周围注射可乐定对CCI大鼠促炎性细胞因子表达的影响_临床医学论文 【摘要】 目的：观察损伤坐骨神经周围注射可乐定对慢性坐骨神经结扎模型(chronic constriction injury，CCI)大鼠促炎性细胞因子表达的影响，探讨α2-肾上腺素受体激动剂是否对已经形成的神经病理性疼痛产生作用。方法：雄性SD大鼠32只，随机分为4组，每组8只。Sham组：只分离皮肤、肌肉，不结扎坐骨神经;Sal组：形成CCI后注射生理盐水组;Clo组：形成CCI后注射可乐定组;Clo+Yo组：形成CCI后注射可乐定和育亨宾组。在注射药物3 d后急性处死动物，取患侧脊髓L4、L5、L6背根神经节采取全自动化学发光法检测TNF-α和IL-6浓度。结果：术后从7 d开始CCI大鼠脊髓背根神经节TNF-α和IL-6浓度有明显增高。损伤坐骨神经周围注射可乐定能明显抑制CCI引起的脊髓背根神经节TNF-α和IL-6的表达(0.01)。结论：损伤的坐骨神经周围注射可乐定可以降低促炎性细胞因子的表达。 【关键词】 可乐定;外周注射;CCI大鼠;神经病理性疼痛;TNF-α;IL-6 　　Abstract　Objictive: To assess the effect of perineural clonidine on the expression of TNF-α and IL-6 in the rats with chronic constriction injury(CCI) in the sciatic nerve. Methods：Thirty-two male SD rats were randomly divided into four groups(18 for each):sham group, Sal group (CCI+ saline), Clo group(CCI+clonidine) and Clo+Yo group(CCI+clonidine+ yohimbin). The rats were killed 3 days after the injection of medicine mentioned above and spinal cord DRG of L4,L5,L6 were taken for determining the concentration of TNF-α and IL-6 of DRG in the rats with automatic luminescent technique. Results: The rats of CCI group demonstrated steady mechanical and heat hyperaglsia from the seventh day after the operation. There was a remarkable increase in the concentration of TNF-a and IL-6 in DRG from the 7th day after the operation. Perineural injection of clonidine could suppress the expression of TNF-α and IL-6 caused by CCI in the DRG of CCI rats(0.01). Conclusion: Perineural injection of clonidine modulates proinflammatory cytokines expression in CCI rats. 　　Key words　Clonidine; Perineural inject; CCI; Neuropathic pain; TNF-α; IL-6 　　神经病理性疼痛(neuropathic pain)是指由于神经系统受到损伤或产生病变而导致的疼痛。病理生理学特点主要表现为痛觉的高反应性,如痛觉过敏( hyperalgesia)、痛觉超敏(allodynia)及自发疼痛( spontaneous pain)等。神经病理性疼痛不同于由于组织损伤而导致的急性疼痛,通常呈慢性，可持续数天或数月,是目前临床治疗的难点之一。可乐定( clonidine)是α2-肾上腺素受体激动剂,临床上常用来治疗高血压。近年来发现它具有很好的缓解神经病理性疼痛的作用，多用于鞘内、静脉注射或伍用其他药物治疗慢性疼痛。本实验拟在经典的神经病理性疼痛模型CCI上，通过在结扎坐骨神经产生痛敏后，在损伤神经周围注射可乐定，探索外周注射可乐