垂体腺苷酸环化酶激活肽对大鼠脑缺血再灌注后诱导性一氧化氮合酶表达的影响_临床医学论文.docVIP

垂体腺苷酸环化酶激活肽对大鼠脑缺血再灌注后诱导性一氧化氮合酶表达的影响_临床医学论文 垂体腺苷酸环化酶激活肽对大鼠脑缺血再灌注后诱导性一氧化氮合酶表达的影响_临床医学论文 【摘要】 目的 探讨大鼠局灶性脑缺血再灌注时氧化氮合酶（iNOS）在PACAP脑保护机制中的作用。方法 采用大脑中动脉线栓法建立局灶性脑缺血再灌注模型，对36只大鼠随机分为假手术组、缺血再灌注组和PACAP组，在大鼠脑缺血2h后进行24h、48h再灌注，应用免疫组化法检测脑组织iNOS的表达。结果 脑缺血再灌注后，与假手术组比较，iNOS的表达量显著增加，阳性细胞数分别为60.02±4.23、80.36±6.24，0.01；应用PACAP后与缺血再灌注组比较，iNOS表达的峰值明显降低，分别为30.47±5.24、40.56±4.84，0.01。结论 PACAP能抑制大鼠局灶性脑缺血再灌注时iNOS的表达，这可能是其脑保护作用之一。 【关键词】 PACAP；脑缺血再灌注；iNOS Effect of PACAP on the expression of iNOS in rats during transient focal cerebral ischemiareperfusion Lu Wenge,Bai Hongying.The First People’s Hospital of Shangqiu City,Shangqiu 476000,China【Abstract】 Objective To investigate the effect of PACAP on the activation and the expression of iNOS in cerebral cortex during transient focal cerebral ischemias the probable mechanism of its protective effect.Methods Thirtysix SD rats were randomly divided into 3 equal groups: sham operation group undergoing sham operation; ischemia/reperfusion(I/R) group undergoing thread embolism of the left middle cerebral artery occlusion(MCAO) to cause focal ischemia for 2 hours and then undergoing reperfusion; and PACAP group undergoing peritoneal injection of PACAP half hour after the ischemiareperfusion of MCAO. Then the rats in the 3 groups were redevided into subgroups of 6 rats, to be decapitated 24，48 hours after reperfusion for the later two groups, and their brains were taken out and fixed. Immunohistochemistry was used to detect the expression of iNOS in the brain.Results The expression values of iNOS in the ischemic cortexes were significantly higher than those in the cortex of the sham operation group(0.01)，and were all significantly lower in PACAP group than those in the I/R group(all 0.01).Conclusion PACAP inhibits the activation and the expression of iNOS during focal ischemiareperfusion which may be one of the mechanisms of its neuroprotective function. 　　【Key words】 PACAP；Ischemiareperfusion；iNOS 　　研究表明,在脑缺血再灌注损伤过程中,NO的生成是重要的发病环节,引起细胞死亡或凋亡