原生质体融合提高非致病性尖镰孢Fo47抗药性.pdfVIP

墨北蓐让学报2011，20(1)：170-173 Acta Boreali-occidentalis Agriculturae Sinica 原生质体融合提高非致病性尖镰孢F047抗药性 宋赵依，金雪菲，张文婷，宗兆锋 (西北农林科技大学植保学院，陕西杨凌712100) 摘要：选择紫外灭活和热灭活亲本原生质体为遗传标记，以非致病性尖镰孢F047和对西瓜枯萎菌有抑制 作用的植物内生放线菌SG2、生长较快并对尖孢镰孢萎蔫专化型有抑制作用的重寄生链霉菌PR进行跨界原 生质体融合，经筛选获得4株融合子F-8、F-9、F-10和F-12，皿内测定结果表明，72h后融合子生长速率显著 快于亲本F047(P一0．05)；抗多菌灵的临界质量浓度分别达到3、9、6、6mg／I。，均高于F047的2mg／L。 关键词：原生质体融合；F047；抗药性；生物防治 中图分类号：Q939．13+2 文献标志码：A 文章编号：1004—1389(2011)01—0170—04 of Resistanceto ImprovementFungicide Non—pathogenic Fusarium StrainF047 Fusion oxysporum byProtoplast SONG Xuefei，ZHANGandZONG Zhaoyi，JIN Wenting Zhaofeng ofPlant AF Shaanxi (College Protection，NorthwestUniversity，Yangling712100，China) Abstract：Selecting withheat—andultravi01et—inactivationas parentalprotoplasts geneticmarkers。pro— fusionof wasconducted Fusarium Strain toplast inter-kingdom amongnon-pathogenic oxysporum SG2withtheinhibitionof ofFusarium F047，endophyticactinomyces growth oxysporumf．sp．hive- usand strainPRwiththeinhibitionof ofFusarium hyperparasite growth oxysporumf．sp．vasinfec— tumand and obtainedafter highgrowingability．Fourrecombinants，F_8，F-9，F-10F-12，were sepa- and rationselection．Theratesofthemwere fasterthan strainF047in growth significantly parental vitroafter72 hours(P一0．05)andthetolerancelevelstoCarbendazimwere3，9，6，6