薄层扫描法测定油茶粕蛋白水解物的多肽.pdfVIP

98 薄层扫描法测定油茶粕蛋白水解物的多肽 朱  培，钟海雁*，黄卫文，周  波，龚吉军，赵清洁  （1 中南林业科技大学食品科学与工程学院，长沙 410004 ；2 粮油深加工与品质控制湖南省 重点实验室，长沙 410004 ；）  摘要：为了研究薄层扫描法测定油茶粕固态发酵蛋白水解物中多肽的含量的可行性，在确定 展开剂及描述波长的基础上，测定了该方法的重复性、加样回收率，并与高效液相色谱法进 行了含量的的对比。结果表明：以谷胱甘肽为标准品、茚三酮为显色剂，样品液中多肽的含 量为1.82 mg/mL，而原料中多肽含量只有0.89 mg/mL，该方法重复性试验标准偏差为0.03% ； 回收试验的平均回收率为106.94%，标准偏差为0.058%，重现性和加样回收率等均符合含量 测定要求。该法测定的含量与反向高效液相色谱法相比无显著性差异(P0.05)。薄层扫描法 适合于在快速检测多肽中应用。  关键词：薄层色谱；油茶粕；固态发酵；蛋白水解物；多肽  中图分类号：S377   文献标志码：A      Determination of Polypeptide in the Protolysate of the Oil-tea Camellia Meal by TCL-Scanning Zhu Pei1,2,3, Zhong Haiyan 1,2,3 , Zhou Bo1,2,3, Gong Jijun1,2,3, Zhao Qingjie1,2,3 1 ( National Engineering Laboratory of Rice and by-products Processing, Changsha 410004; (2Hunan Province Key Laboratory of Grain ＆Oil Processing and Quality Control, Changsha 410004; (3School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004) Abstract: The aim of this paper was to determine the feasibility of TCL-scanning in analysis of the content of polypeptide in the protolysate of oil-tea camellia meal. Based on the determination of developers and wavelenghth, the repeatation and recovery of this method was tested. And then quantitative result was compared with that of HPLC. Results showed that the content of polypeptide was 1.82 mg/mL in the protolysate but 0.89 mg/mL in raw materials when using glutathione as standard and monohydrate as chromogenic agent, and the method had a high accuracy and precision with 0.03% of relative standard deviation (RSD) in repeatation test and 0.058% of RSD and 106.94% of recovery in recovery test. There were no significant differences in content compared with that of HPLC(p0.05).