大孔吸附树脂对必特螺旋霉素分离纯化.pdfVIP

大孔吸附树脂对必特螺旋霉素的分离纯化* 徐婕，朱家文*，陈葵 （华东理工大学化学工程研究所，上海 200237 ；华东理工大学化学工程研究所，上海 200237 ；华东理工大学化学工程研究所，上海 200237 ） 摘要:本文研究了采用大孔吸附树脂，利用选择吸附的原理分离提纯必特螺旋霉素的工艺过程。对比了 HZ806 、 HZ816 、 HZ820 、 HZ830 、 XAD16 和 SP207 六种大孔吸附树脂对必特螺旋霉素的吸附性和选 择性，结果表明，非极性树脂HZ820 为较适合的树脂。 通过间歇吸附平衡实验，研究了HZ820 树脂对必特螺旋霉素的吸附相平衡，Langmuir 和Freundilich 吸附等 温线方程均较好地描述了必特螺旋霉素在HZ820 树脂上的吸附相平衡关系；通过间歇吸附动力学实验，采 用数学模型模拟HZ820 对必特螺旋霉素的吸附量和时间的关系；通过固定床连续实验，采用HZ820 为吸 附剂，优化了吸附和解吸条件。在前述研究的基础上，采用较优的实验条件，经过固定床吸附和解吸过程， 吸附过程中必特螺旋霉素的各组分表现出了明显的竞争吸附特性；异戊酰螺旋霉素I 、II 、III 的质量含量从 80.4%增大到88.7%；异戊酰螺旋霉素III 的质量含量从41.7%提高到59.0%，产品收率可到75.0%. 关键字：吸附，解析，必特螺旋霉素，大孔树脂 Enrichment and purification of bitespiramycin with macroporous resins Jie Xu, Jiawen Zhu*, Kui Chen (Chemical Engineering Research Centre, East China University of Science and Technology, Shanghai 200237, China) Abstract : The selective adsorption has been chosen to purify bitespiramycin in this paper. The adsorption and desorption properties of bitespiramycin on six different macroporous resins (HZ806, HZ816, HZ820, HZ830, XAD16 and SP207) have been compared systematically. According to the adsorption capacity and selectivity of 4’’-O-isovalerypiramycin, HZ820 is the most suitable resin. The equilibrium data on HZ820 in a batch system has been well described by Langmuir and Freundlich models. Dynamic adsorption and desorption experiments have also been performed by column packed with HZ820 to optimize the separation process of 4’’-O-isovalerypiramycin from aqueous solution. After being treated with HZ820, the content of 4’’-O-isovalerypiramycin has increased from 80.4% to 88.7%, the content of 4’’-O-isovalerypiramycin III has increased from 41.7% to 59.0%, and the percent