一株有机磷农药降解菌的分离_鉴定与降解酶基因的克隆_王圣惠.pdfVIP

2007, 26(): 84- 88 J ournal of A g ro-Env ironm ent S cience 1, 3 2 1, 3 1, 3 王圣惠 , 闫艳春 , 徐刚明 , 李莹莹 ( 1., 271018; 2. , 100081; 3. , 271018) : Yw18, ,, (Ochrobacterum sp. ) Yw18 - 1 - 1 , , 0. 5 h 50 mg# L 90% , 8 h 50 mg# L , PCR mpd : ; ; ; : X 172 : : 1672- 2043( 2007)- 0084- 05 Isolation, Identification and C lon ing m pd Gene of a S train ofM ethylparath ion- D egrad ing M icoor- gan ism s 1, 3 2 1, 3 1, 3 W NG Sheng- hui , Y N Yan- chun, XU Gang-m ing , L IY ing- ying ( 1. College ofL ife Science of Shandong griculturalUniversity, Taian 271018, China; 2. Graduate SchoolofChinese cade- my of griculturalSciences, Beijing 100081, China; 3. StateKey Laboratory ofCrop Biology Shandong, Taian271018, China) A bstrac t: strain ofmethylparathion- degrading microorganisms named asYw18 in our labwas isolated from activated sludge polluted by organophosphorus pesticides for a long- terms. Ithas been indicated thatYw 18 could utilize methylparathion andQ - nitrophenol, a mainmetabolite product for the insecticide, as sole source of carbon and itwas identified asOchrobacterum - 1 sp, and capable of degrading 50mg# L ofmethylparathion, Q- nitrophenol, rapidly in 0. 5 h and 8 h respectively, after ana- lyzingmetabolite products with gas chromatography ( GC) and spectrophotometry. t the same tmi e, phylogenetic analysis of Yw18was carried out, and thempd gene located in chromosomewas coloned by PCR in our lab. K eyw ords: methylparathion; Q- nitropheno;l biodegradation; hydrolase gene ( Organophosphate, OPS) ,, , , , , , (M eth