基因芯片检测PTA石化废水生物分子毒性.pdfVIP

基因芯片检测 PTA 石化废水生物分子毒性 1 1 1 1 1 2 1 朱程军 ，郎有哲 ，张徐祥 ，孙石磊 ，赵大勇 ，顾继东 ，程树培 （1.南京大学环境学院，南京210093； 2.香港大学生态学与生物多样性系，特别行政区，中国香港） 摘 要：应用基因芯片（Mouse Genome 430A 2.0）检测精对苯二甲酸(PTA)石油化工废水对雄性小鼠 肝脏基因表达的分子毒性。小鼠体重平均为 20g，经口腔灌胃 PTA 剂量为 0.0044g/（Kg•d）35d。结 果是 232 个基因的表达增强，74 个基因的表达减弱。表达增强的或表达减弱的前 5 个基因，均涉及 小鼠的免疫功能、糖等代谢功能、生物膜功能、神经功能等。检测 PTA 纯化学品分子毒性其它方法的 毒性效应剂量，是基因芯片检测方法的 37-2270 倍。基因芯片检测方法对于预警环境健康和生态安全， 具有灵敏、快速、高效的特征。 关键词：基因芯片；PTA 废水；雄性小鼠；分子毒性；效应剂量 PTA Wastewater Toxicity Detected with DNA Microarray 1 1 1 1 1 2 Zhu Cheng-jun , Lang You-zhe , Zhang Xu-xiang , Sun Shi-lei , Zhao Da-yong , Gu Ji-dong , 1 Cheng Shu-pei (1.School of the Environment, Nanjing University, Nanjing 210093; 2.Department of Ecology and Biodiversity, University of Hongkong, Hongkong, SAR China) Abstract: Purified terephthalic acid (PTA ) wastewater molecular toxicity to the male mice (Mus musculus ， KM) detected by use of DNA microarray （Mouse Genome 430A 2.0 ）was conducted. The dose of PTA was 0.0044 g/Kg to each male mouse (AW 20g) through feeding 0.2mL /d of the raw wastewater for 35d. There were 232 gene expression level higher and 74 gene expression level lower obviously than the controls discovered on Mouse Genome 430A 2.0 hybridized with the mice liver cRNA samples. The first 5 genes for broth expression level higher and expression level lower involve the function systems of immunity, sugar metabolism, membrane and nerve. The dose of pure chemical PTA for molecular toxicity occurred was 37-2270fold as high as that in the wastewater. The data suggested that DNA microarray technique is more sensitive, effective and rapider than other measu