脑源性神经营养因子载体细胞治疗新生大鼠低氧缺血性脑损伤阿片机制.pdfVIP

# 318 # 2002 10 115 Chin J Emerg Med, October 2002, Vol1 11, No15 ## 洪新如 郑铃 侍坚 陈新民 = B- L (BDNF) (HIBI) 7d HIBI+ BDNF ( A) HIBI+ BDNF+ DAMGO ( B) HIBI (C) (D) HIBI, BDNF (AB) (C) , L DAMGO ( 012Lg, B) , 013d B- ( ir-B-%1) 1d (MDA) ( 1) ir-B-%1 , A C (0d) , BC D ( 01d) , ABC D (0d) , C A D ( 1d) ( 2) C AB D , MDA ABC D , A B C (3) , BC D , C A B , A B ; ABC D , A C B-HIBI BDNF HIBI , B- L HIBI = ; ; ; Opioid mechanism in the treatment of hypoxic-ischemic brain injury by intracerebral transplantation of genetically modified myoblasts producing brain-derived neurotrophic factor in neonatal rats HONG Xinru, ZHENG Ling , SHI Jian, et al. Pediatrics Centre of PLA , Department of Pediatrics, uzhou GeneralHosp ital , uzhou 350025, China = Abstract Obj ective o explore the role of B-endorphin and opioid L receptor on the experimental treatment of hypoxic-ischemic brain injury ( HIBI) by intracerebral transplantation of genetically modified myoblasts expressing and secreting brain-derived neurotrophic factor ( BDNF) in neonatal rats. Methods Seven-day-old Sprague-Dawley rats were randomly divided intoHIBI+ BDNF group (A) , HIBI+ BDNF+ DAMGO group ( B) , HIBI group ( C) and sham-operated group ( D) . Pups were transplanted unilaterally intracerebroparenchymally with either genetically modified myoblasts 4 producing and secreting BDNF (A, B) or their parent cells ( C) at 01 8Ll (4 @ 10 / Ll) followed by a cerebroventricular microinjection of opioidL receptor agonist DAMGO ( 012Lg, B) or vehicles (A, C) shortly after HIBI undergone by a permanent ligation of left common carotid artery followed by a 21