免疫组化或荧光图片在PHOTOSHOP中叠加.pdfVIP

免疫组化或荧光图片在PHOTOSHOP 中的 叠加 2008-08-10 19:08 This protocol works well if you have images of a double or triple fluorescently stained structure that you wish to represent with 2 or 3 different colors, respectively. It is important that all the images were taken with the same objective and positioning to allow for proper overlay and alignment of images during the colorizing process. 1. Open up Adobe PhotoShop. Go to File and Open all of the desired black and white images you obtained from the confocal or the fluorescence microscope that you would like to represent in t