中药复方对糖尿病大鼠坐骨神经氧化应激与超微结构影响.pdfVIP

1) 首都医科大学中医药研究生论坛(201 中药复方对糖尿病大鼠坐骨神经 氧化应激及超微结构的影响 北京中医药大学东方医院 邹大威 【摘要】目的：探讨中药复方糖络宁(TLN)对糖尿病大鼠坐骨神经氧化应激及超微结 构的影响。方法：SD大鼠55只，随机选取12只为正常对照组，余43只大鼠予一次性腹腔 注射STZ建立糖尿病(DM)大鼠模型，DM造模成功后随机分为模型组、糖络宁组、Q硫辛 酸组。两给药组干预4个月后，用透射电镜观察各组大鼠坐骨神经的超微结构，检测坐骨神 组相比无差异仔】O．05)。透射电镜观察坐骨神经超微结构显示模型组部分纤维髓鞘增厚或 松解，轻度轴索变性。糖络宁组仅见少数纤维髓鞘松解，病变明显轻于模型组。结论；中药 复方糖络宁可能通过提高SOD、CAT的活力、清除脂质氧化终产物MDA，减轻糖尿病周围神 经病变(DPN)的过氧化损伤，减少坐骨神经细胞凋亡，延缓DPN的进展。 【关键词】糖尿病神经病变；氧化应激；超微结构：中药复方 ofTraditionalChineseMedicineonOxidative Effects StressandUltrastructureofDiabeticRatsSciaticNerve 【Abstract1 To theeffectsofT豫ditionalChinesemedicineOn Objectiveinvestigate oxidativestressandultrastructureofdiabeticratssciaticnerve．MethodThe55ratsweredivided intocontrol the 43 ratsof 2，and experimental experimental groupl grouprandomly．The group were one-time ofSTZtobuilddiabeticratsmodel．Therats given intraperitonealinjecfion were dividedintothemodel Luo modelingsuccessfullyrandomly group，TangNinggroup(TLN) and acid interventionwithTLNorLAfor4months，the alpha-lipoicgroup(LA)．After nerve each ultrastructureofsciatic in wereobservedtransmissionelectron group by the detectedtheMDAcontentandthe of and sametime，we microscopy．At vitalitySOD，CAT insciaticnerve．Resultswiththecontrol contentofMDAandthe Caspase3 Compared group，the of wereincreasedinmodel insciatic SOD,CAT vitalityCa