TF1细胞凋亡相关基因的研究.pdfVIP

生物化学与生物物理进展990112 生物化学与生物 物理进展 PROGRESS IN BIOCHCMISTRY AND BIOPHYSICS 1999年 第1期 No.1 1999 TF-1细胞凋亡相关基因的研究 刘红涛　王玉刚　张颖妹　宋泉声　敬保迁　袁　勇　马大龙 摘要　利用近年来发展起来的代表差异分析（cDNA representational differences analysis, cDNA-RDA ）技术研究了在人红白血病细胞株TF-1细胞撤除细胞因子后进入 凋亡时诱导表达的基因.发现了6个新基因片段.其中有三个经与GenBank nr和dbEST查 询均没有发现同源性，已经向GenBank进行登记，登记号分别为U83208 ，U83279 ， U83397.此外还发现一批已知基因的表达与凋亡相关，其中包括Hou和人硫氧还原蛋白 等, 提示它们在凋亡中可能起作用.这项工作为进一步研究凋亡相关基因打下了良好基 础.通过RDA 的研究结果，有可能发现人白血病细胞凋亡的特异标记蛋白或发挥作用的 重要蛋白，以期为白血病治疗提供理论基础. 关键词　TF-1细胞株，代表差异分析，凋亡 学科分类号　R392.1 Studies on the Apoptosis-Related Genes of TF-1 Cell Line by cDNA-RDA Technique. LIU Hong-Tao, WANG Yu-Gang, ZHANG Ying-Mei, SONG Quan-Sheng, JING Bao-Qian, YUAN Yong, MA Da-Long (Department of Immunology, Beijing Medical University, Beijing 100083, China). Abstract　The genes effecting in the process of the apoptosis of TF-1 cell line when it is deprived of the cytokine in the culture medium were studied by RDA (representational difference analysis) method. The TF-1 cell depriving of cytokines for 8 hours was selected as the Tester and normal-cultured TF-1 cell as the Driver. Seven gene fragments were found uniquely expressed or highly expressed in the process of apoptosis of TF-1 cell line which include some known genes such as Hou and thioredoxin that formerly suggested to play a role in apoptosis.There are three fragments are complete novel after searching the nr and EST catalogues of GenBank and were banked into GenBank. The accession numbers for them are U83208, U83279, U83397 respectively. From the novel gene fragments, the complete cDNA sequence of them can be fished and the bioactivity and function