急性肾缺血再灌注大鼠肾细胞游离钙水平与细胞凋亡的研究.pdfVIP

2010 7 14 7 999 : 1007- 4287(2010) 07- 0999- 03 * 赵 军, 张文岚 , 孙 玲 ( , 130021) 2+ : ( [ Ca ] i) , , , Fura2/ AM [ Ca2+ ] i , Ca2+ , ( P 0.01) ; , ( P 0. 05P 0. 01) , ( [ Ca2+ ] i) , , Ca2+ , , : ; ; ; : R 92 :A Studies of both intracellular ionized calcium level and apoptosis during acute renal ischemic reperfusion injury ZHAO Jun, ZHANG Wenlan, S UN Ling, et al. ( The Department of Urolog , Organ Transplantation Center the First H ospital , Jilin Universit , Changchun 130021, China) Abstract: Objective Effects of renal ischemic reperfusion injury on intracellular inonized calcium level and apoptosis are ob serverd.Methods The model of rat s acute renal ischemic reperfusion injury was established by both cutting the left kidney and oc clusing right kidney pedicle.The renal intracellular [ Ca2+ ] i level and the renal cell apoptosis were measured by using Fura2/ AM fluorescence assay and flow cytometry respectivly. Results Compared with the control group, renal intracellular [ Ca2+ ] i level in creased significantely ( P 0.01) and apoptosis rate increased significantely ( P 0.05, P 0. 01) at different periods of ischemic reperfusion. Conclusion At different periods of rat s acute renal ischemic reperfusion injury, renal intracellular [ Ca2+ ] I rises and becomes postive relationship with the apoptosis rate.There is positive relevent between the reperfusion time and renal intracellular [ Ca2+ ] i overloading.This may be important reason of renal disfunction due to reperfusion injury. Key words: ischemic reperfusion; intracellular ionized calcium; renal tissue; apoptosis