条件复制腺病毒携带白细胞介素24增强 Hela 细胞放射敏感性.pdfVIP

现代肿瘤医学 2015年05月 第2 卷第9期 MODERN ONCOLOGY，May.2015，VOL.2 ，NO.09 ·1205 · 条件复制腺病毒携带白细胞介素24增强 Hela细胞放射敏感性 陈亚平 ，姜晓晓 ，冯守信 ，蒋 冠1 2 4 Ａ ｃｏｎｄｉｔｉｏｎａｌｌｙ ｒｅｐｌｉｃａｔｉｎｇ ａｄｅｎｏｖｉｒｕｓ ｃａｒｒｙｉｎｇ ｉｎｔｅｒｌｅｕｋｉｎ -24 ｉｎｃｒｅａｓｅｄ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｒａ- ｄｉａｔｉｏｎ ｏｎ Ｈｅｌａ ｃｅｌｌｓ 1 2 4 Chen Yaping ，Jiang Xiaoxiao ，Feng Shouxin ，Jiang Guan 1Department of Oncology；Department of Urology；Department of Stereotactic Branch，Affiliated Hospital of Xuzhou Medical College，2 JiangsuXuzhou221000，China；Department of JiangsuProvinceKey Laboratory of Biological Cancer herapy，JiangsuXuzhou221000，4 China. 【Abstract】 Objective：To evaluate the effects of the conditionally replicating adenovirus carrying interleukin-24 combinedwith radiation on cervical cancer Hela cell.Methods：Hela cellswere divided intofour groups：PBS group， radiation group，ZD55-IL -24 group and ZD55 -IL -24 with radiation group.The cell viability was assessed by MTT assay.The apoptotic rate of cells was assessed by Annexin V -FITC/ PI assay.The expression of Bax，Bcl -2 was detectedby Westernblot.Results：PBSgroup，cellviability of radiationgroup，ZD55-IL-24 group andZD55- IL-24 with radiationgroupwere（7.28±1.99）%，（45.16±4.12）%，（ .2 ±2.6 ）% and（85.27±2.0 ）%， which revealscellviability of ZD55-IL-24 withradiationgroupwashigherthanother groups（P0.05）.Annexin V -FITC/ PI revealed that the apoptotic rate of ZD55 -IL -24 with radiation was higher than other groups （P 0.05）.ROSlevelofZD55-IL-24withradiationwashigherthanothergroups（P0.05）.Theexpressionof Bax in ZD55-IL-24 withradiationgroupwashigherthaninothergroups（P0.05），whiletheexpressionof Bcl-2 in ZD55-IL-24 withradiation groupwaslower than in other groups（P0.05）.Conclusion：The conditionally repli- cating adenovirus carrying interleukin-24 can increase radiosensitivity of cerv