常用缓存溶液配制方法.docVIP

常用1.甘氨酸-0.05M）X ml 0.2M甘氨酸 +Y ml 0.2M 再加水稀200mlpH X/ml Y/ml pH X/ml Y/ml2.2 50 44.0 3.0 50 11.42.4 50 32.4 3.2 50 8.22.6 50 24.2 3.4 50 6.42.8 50 16.8 3.6 50 5.0甘氨酸分子量＝75.07 0.2M甘氨酸溶液含15.01g/L2.-盐酸缓冲液（0.05M）X ml 0.2M +Y ml 0.2M盐酸 再加水稀200mlpH X Y pH X Y2.2 5 4.670 3.2 5 1.4702.4 5 3.960 3.4 5 0.9902.6 5 3.295 3.6 5 0.5972.8 5 2.642 3.8 5 0.2633.0 5 2.032 ? 邻苯二甲酸氢钾分子量＝2.4.23 0.2M邻苯二甲酸氢钾溶液含40.85g/L3.磷酸-柠檬酸缓冲液pH 0.2M Na2HPO4/ml 0.1M柠檬酸/ml pH 0.2M Na2HPO4/ml 0.1M柠檬酸/ml2.2 0.40 19.6 5.2 10.72 9.282.4 1.24 18.76 5.4 11.15 8.852.6 2.18 17.82 5.6 11.60 8.402.8 3.17 16.83 5.8 12.09 7.913.0 4.11 15.89 6.0 12.63 7.373.2 4.94 15.06 6.2 13.22 6.783.4 5.70 14.30 6.4 13.85 6.153.6 6.44 13.56 6.6 14.55 5.453.8 7.10 12.90 6.8 15.45 4.554.0 7.71 12.29 7.0 16.47 3.534.2 8.28 11.72 7.2 17.39 2.614.4 8.82 11.18 7.4 18.17 1.834.6 9.35 10.65 7.6 18.73 1.274.8 9.86 10.14 7.8 19.15 0.855.0 10.30 9.70 8.0 19.45 0.55Na2HPO4分子量＝141.98 0.2M溶液含28.40g/LNa2HPO4·2H2O分子量＝178.05 0.2M溶液含35.61g/LC6H8O7·H2O分子量＝210.14 0.1M溶液含21.01g/L4.柠檬酸-氢氧化钠-盐酸缓冲液pH 钠离子浓度/M 柠檬酸C6H8O7·H2O/g 氢氧化钠NaOH/g 浓盐酸HCl/ml 终体积/L2.2 0.20 210 84 160 103.1 0.20 210 83 116 103.3 0.20 210 83 106 104.3 0.20 210 83 45 105.3 0.35 245 144 68 105.8 0.45 285 186 105 106.5 0.38 266 156 126 10使用1g酚，若最后pH有50%氢氧化钠溶液或浓盐酸调节，冰箱保存。柠檬酸-柠檬酸钠缓冲液（0.1M）pH 0.1M/ml 0.1M柠檬酸钠/ml pH 0.1M柠檬酸/ml 0.1M柠檬酸钠/ml3.0 18.6 1.4 5.0 8.2 11.83.2 17.2 2.8 5.2 7.3 12.73.4 16.0 4.0 5.4 6.4 13.63.6 14.9 5.1 5.6 5.5 14.53.8 14.0 6.0 5.8 4.7 15.34.0 13.1 6.9 6.0 3.8 16.24.2 12.3 7.7 6.2 2.8 17.24.4 11.4 8.6 6.4 2.0 18.04.6 10.3 9.7 6.6 104 18.64.8 9.2 10.8 ? C6H8O7·H2O分子量＝210.14 0.1M溶液含21.01g/LNa3C6H5O7·2H2O分子量＝294.12 0.1M溶液含29.41g/L6.乙酸-乙酸钠缓冲液（0.2M）pH 0.2M NaAc/ml 0.2M HAC/ml pH 0.2M NaAc/ml 0.2M HAC/ml3.6 0.75 9.25 4.8 5.90 4.103.8 1.20 8.80 5.0 7.00 3.004.0 1.80 8.20 5.2 7.90 2.104.2 2.65 7.35 5.4 8.60 1.404.4 3.70 6.30 5.6 9.10 0.904.6 4.90 5.10 5.8 9.40 0.60NaAc分子量＝136.09 0.2M溶液27.22g/L7.磷酸(1)