糖鞘脂Gb3的配体结构和配体胞内运输的关系.pdfVIP

体配体仍旧定位于高尔基体。进一步的研究表明突变体无法到达内质网，在到达高尔基 体后降解。表明与受体的结合强弱影响了到内质网的运输，即STxB的胞内运输依赖于 STxB．Gb3复合物的稳定作用。 自然界存在的Gb3配体，而Gb3抗体38．13可以看成是人工合成的配体，两种配体都结 合Gb3的寡糖部分。根据结果，Gb3抗体38．13能结合于细胞膜表面，但无法进入细胞 诱导产生的，而并非利用了细胞内固有的Gb3转运途径。 关键词：糖鞘脂Gb3；志贺毒素B亚基；配体结构；胞内运输；突变体；内质网； 高尔基体 II Abstract The isenrichedinthe glycosphingolipidglobotriaosylceramide(Gb3)，whichplasma membraneofmammalian an roleincell cells，playsimportant and transduction．Gb3isthecellular of recognition signaling receptorShigatoxin(STx)， whichis toxinis oftwo of producedbySh／ge／tadysenteriae．Shigacomposedmoieties，one a thatinhibits which(Asubunit，STxA)carriesdeadenylaseactivity proteinbiosynthesis by ontheribosomal28S theother the acting rRNA，whereasone(B ofthetoxintothe Gb3．Inmammalianhasbeenshownthat binding receptor cells，it Shiga toxinor toxinB the routefromthe Shiga subunit(STxB)followsretrogradetransport plasma membranetothe the endosome endoplasmicreticulum(ER)，viaearlyendosome／recyling the the environmentofthelate (EE／RE)andGolgiapparatus，circumventingdegrading otherstudiesrevealthat labeledGb3addedintocells endocyticpathway．And fluorescently can also tothe evidencehas thatthere target Golgiapparatus．However,availablesuggested betwodifferent routes