乳铁蛋白特性评价与的研究.pdf

摘 要 乳铁蛋白是一种具有多种生理功能的天然活性糖蛋白，最先在乳汁中发现，后 来研究表明，它广泛存在于动物体的泪液、唾液、汗液、胰液、胆汁、精液等各种 分泌液中。乳铁蛋白及其水解产物乳铁蛋白活性多肽具有明显的抗菌、调节免疫、 促进铁的吸收等多种生理功能。乳铁蛋白可与多种抗生素及抗真菌制剂协同作用， 可以更有效地治疗疾病；乳铁蛋白能调节体内铁的平衡，调节骨髓细胞生成，促进 细胞的生长；能抑制人体肿瘤细胞的生长，预防及治疗肿瘤。目前，将乳铁蛋白作 为一种全新营养素的研发已成为热点。 本课题研究了乳铁蛋白性能参数与其生物活性之间的关系。以目前针对乳铁蛋 白研究中较为成熟的铁饱和度，未变性乳铁蛋白含量为参数，还额外增加了脂多糖 含量作为评价参数。虽然还没有标准将脂多糖作为食品原料品质好坏的鉴定指标， 但脂多糖含量可以反映食品生产环境及生产过程中的洁净程度，也可以反映产品的 品质。通过乳铁蛋白对大肠杆菌的抑菌试验及对有益菌的促菌试验，分析各类指标 与乳铁蛋白生物活性之间的关系，确定高品质乳铁蛋白的评价指标。 本试验在乳铁蛋白抑菌性试验方法的筛选过程中，比较了牛津杯法，平板菌落 计数法及测定菌液OD 值法的抑菌效果，并认为牛津杯法不适用于乳铁蛋白抑菌效 果的测定。通过单因素试验和多因素对照试验，寻找影响乳铁蛋白生物活性的因素。 乳铁蛋白的含量，越高越好；脂多糖含量，越低越好；乳铁蛋白铁饱和度虽然是乳 铁蛋白一项重要的理化指标，但乳铁蛋白的铁饱和度与其抑菌和促菌活性间不存在 规律性。 关键字：乳铁蛋白；铁饱和度；脂多糖；性能评价 Evaluation and Research on the Characteristics of Lactoferrin Abstract lactoferrin(LF) is an iron-binding glycoprotein ,and it has various biological function. LF mainly exists in numerous exocrine secretions of mammals . Such as, tears, saliva, sweat, and pancreatic juice, bile, semen and so on, it was first found in milk. LF and its hydrolysis product, Lactoferricin, not only have broad spectrum of antimicrobial properties, but also have the ability to improve antiviral activity, and anticancer activity, and immuno-regulatory anctivity of the body to prevent and cure infectious disease . LF has synergic effect in treatment for disease when used together with several antibiotics and antifungal agents.LF can adjust the balance of iron in the body, adjust the marrow cells in differentiation, promote normal cells to grow, while inhibit the growth of tumor cells. At present, the study on LF as a brand-new nutrient has become a hotspot . In this paper,the relationship between biological activity of lactoferrin and its composition, suchi