盐地碱蓬SsNHX1基因的克隆及其转基因拟南芥的培育.pdfVIP

at^一C,}-t5},f,匕-— — 一.一 — 一 一一一一“ Abstract IsolationofSsNHX1fromhalophyteSuaedasalsaandgene engineeringforArabidopsisthaliana Themaladiescausedbysaltstressarisefromthedisurptionofcellularaqueousand ionicequilibrium.Theions,especiallyNaaccumulatesexcessivelyinthecytoplasm,itcan bet6xictothemetabolismandaffecttheregulationmechanismoftheplantcell.Tocope withsaltstress,plantshavedevelopedavarietyofadaptationmechanisms.Oneisthe accumulationofthesoluteosmo-protectants,the,otheris,themechanismsofion homeostasisincludingNa+extursionsystem,orsequesterNa+intothevacuolarandregulate theratioofK+1Na+,especiallyinhalophyte.Na+/H+antiporterplaysaroleinthisprocess AtNHX1(encodingavacuolarNa+/H+antiporter).andSOS](encodingaplasmamembrane Na+/H+antiporter)wereisolatedfromglycophyteArabidopsisthaliana,theover-expression ofAtNHX1inA.thalianaincreasesthesalttoleranceoftransgenicplantssignificantly.The researchwasdone roleoftheSsNHXIinhalophyteSuaedasalsaisionhomeostasis.Further throughtheover-expressioninA.thaliana,perhapsitcouldhelpustoelucidatethea t s l toleranceofhalophyte.WeisolatedSsNHXIrfomhalophyteSsalsaandtransformedn o t i w n e Arabidopsis.GeneengineeringbaseonreduceNa*contentincytoplasmcreatesa approachtoplantsalttolerance.SsNHXIperhapsplaysanimportantroleinregulateNa homeostasisofhalophyteS.salsa,soover-expressionofSsNHXIinA.thalianamaymake progressoftheresearch.Itcanattributetoelucidatetheionhomeostasisofplantcell Inthisexperiment,weisolatedtheSsNHXIgene(GenBbankaccessionnumber: AF370358).FortheNa+/H+antiportergenehadbeenisolatedrfomA.thaliana,Oryzasativa etal..TherewasconservedfragmentindifferentNa/H+antiporter,wedesignedtheprimers accordingtotheconservedsequence,amplifiedtheshortf