第四章 基因重组与定点突变.ppt

第四章  基因重组 与定点突变 3.Co-expression of chaperones and/or foldases. （与分子伴侣或折叠酶共表达） Two classes of proteins play an important role in in vivo protein folding. Molecular chaperones promote the proper isomerization and cellular targeting by transiently interacting with folding intermediates. The best characterized E. coli systems are: GroES-GroEL DnaK-DnaJ-GrpE ClpB GroEL/GroES复合物 GroES GroEL Foldases accelerate rate-limiting steps along the folding pathway. Three types of foldases play an important role: peptidyl prolyl cis/trans isomerases (PPIs肽基脯氨酰异构酶) disulfide oxidoreducta