《Polycomb chromobox 4 enhances migration and pulmonary metastasis of hepatocellular carcinoma cell line MHCC97L.》.pdf

SC C C瓣iNA LifeSciences · RESEARCH PAPER · June2014 Vol57 No．6：610-6l7 doi：lO．1007／sll427—014—4663—9 Polycombchromobox4enhancesm igrationandpulmonary metastasisofhepat0cellularcarcinomacelllineM HCC97L MEIZhu，JIAOHuiKe，WANGWei，LIJie，CHENGuoQiang’＆XUYing Deparmwnt时‘Patltcq)h3siolog3．KeyLaboratoO,啦CellDifferentiationandApoptosis(tMinistlTt(Education,RuijinHowitalShanghai JiaoTangUn ，ersl¨SchoolofMedicine．Shanghai200025．China, 11rstituteofHealthSt。iences．Shanghai]nstitute,s{orBiologicalSc。ienceso{ChineseAc。ademyofSciencesShanghaiJiaoTongUniversiO, SchoolofMedicine，Shanghai200025，China ReceivedM arcia22，2014：acceptedApril3，2014；publishedonlineMay 15．2014 W erecentlyreportthattheexpressionofpolycombchromobox4fCbx41issignificantlycorrelatedwiththeoverallsurviva1of agreatcohortofheDatoceI1u1arcarcinoma(HCC1patientsanditenhanceshypoxia—inducedvascularendothelialgrowthfactor (VEGF1expression and angiogenesisin HCC cellsthrough enhancing sumoylation ofhypoxia jnduciblefactor—lalpha (HIF—lⅡ1．HerewecontinuetoinvestigatethepotentialeffectsofCbx4onthemigrationandmetastasisofthemetastaticHCC celllineMHCC97L．Ourresultsshow thatCbx4overexpressioninthecel1lineincreasestheinvitrovesselformationofvas— cularendothelialcellsii1itsSUMO interactionmotifs—dependentmanner．andpromotesthe iivitromigrationofthecancercell whichcanbeeffectivelyabrogatedbyanti—VEGFantibody．AlthoughCbx4expressiondoesnotimpactthe nvitrogrowthof MHCC97Lcells，jtstillpromotestheprogressionandmetastasisoforthO