《响应面法优化赤霞珠葡萄叶总黄酮的提取工艺 Optimization of Extraction of Total Flavonoids from Cabernet sauvignon Leaves by Response Surface Methodology》.pdfVIP
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《响应面法优化赤霞珠葡萄叶总黄酮的提取工艺 Optimization of Extraction of Total Flavonoids from Cabernet sauvignon Leaves by Response Surface Methodology》.pdf
新疆农业科学2013,50 11 :2037—2045 Sciences XinjiangAgricultural doi:10.6048/j.iasn.1001—4330.2013.11.011 响应面法优化赤霞珠葡萄叶总黄酮的提取工艺 边梅娜1’2,李松泽3,白红进1’2,刘帮磊2,李春2 1.新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室。新疆阿拉尔843300; 2.塔里木大学生命科学学院,新疆阿拉尔843300;3.新疆海瑞盛生物工程股份有限公司,新疆焉耆841loo 摘要:【目的】优化赤霞珠葡萄叶中总黄酮的提取工艺。【方法】以葡萄叶中总黄酮的提取率为指标,考察了 乙醇浓度、液料比、提取时间、提取温度对总黄酮提取率的影响,在单因素试验的基础上,根据中心组合设计, 采用四因素三水平的响应面分析法进行工艺优化。【结果】优化得到总黄酮最佳提取工艺参数为:乙醇提取 h,提取温度80℃,在此条件下赤霞珠葡萄叶中总黄酮的 浓度为56%,液料比为40:1 mL/g ,提取时间2.1 提取率为5.61%。【结论】采用响应面法优化提取赤霞珠葡萄叶总黄酮的工艺条件稳定可行。 关键词:赤霞珠葡萄叶;总黄酮;响应面法:提取工艺 文献标识码:A 文章编号:1001—4330 2013 11—2037一09 中图分类号:R663.1;$609.9 ofExtractionofTotalFlavonoidsfromCabernet Optimization Leaves Surface sauvignonbyResponseMethodology BIAN Chun2 Mei—nal”,LI Bang—lei2,LI Song—ze3,BAIHong—jinl”,LIU and Resources讥TarimBasin Pro— ProtectionUtilization 1.KeyLaboratoryof of ofXinjiang Biological ductionand Construction of印Sciences,TarimUniversity, Cop,Alar觑,批增843300,China;2.College Alar HairuishengBiological Co.,Lid.,YanqiXinjiang Xinjiang843300,China;3.Xinjiang Engineering 841100,China theextraefionoftotalflavonoidsfromCabernet leav- Abstract:【Objective]Tooptimize process sauvignon extraction oftotalflavonoidsfromCSLwastakenas effectsof es CSL .【Method]Theefficiency index,the theethanol material timeandextraction ontheextractionef- concentration,liquidratio,extraction temperature oftotalflavonoidswereexamined.Onthebasisof factor tothe of single design ficiency experiment,according central extractionwas ofthree surface thefourvariables levelsof composite,the optimized response by analy- obtainedas ethanolconcen- conditionsof totalflavonoidswere sis.【Result]The foUows:the optimal extracting trafionwas ratiowas extracfiontimewas2.1 extraction 56%;the h;the liquid—material40:1 mL/g ;the theabove extractionrateofCabernet Leavestotalfla— was80。C.Under conditions,the sauvignon temperature vonoidswas extractionofCSLtotalflavonoidsof surfacemethod· 5.61%.【Conclusion】Theprocess response wasreliable. ology words:Cabernet surface Ke
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