干旱驯化后节水抗旱稻苗期不同发育时间DNA甲基化模式变化分析.pdfVIP

中国水稻科学 (ChinJRiceSci)，2014，28(1)：32—4o httpt}{ ．ricesci．cn 32 DOI：10．3969／j．issn．1001—7216．2O14．01．005 干旱驯化后节水抗旱稻苗期不 同发育时间DNA 甲基化模式变 化分析 郑小国1，2，# 陈亮 ，# 楼巧君 夏辉 李明寿 罗利军 ，， (1上海市农业生物基因中心，上海 201106；2华中农业大学 植物科学技术学院，武汉 430070；#共 同第一作者； 通讯联系人 ， E-mail：lijun@sage．org．cn) ChangesinDNA M ethylationPatterninaW ater——savingandDrought—r‘esistance RiceVarietyatThree——leafandFour——leafstagesafterDroughtDomestication ZHENGXiao—guo，。，#，CHENLiang，#，LOUQiao—jun ，XIAHui，LIMing—shou ，LUOLi—jun’。， (1ShanghaiAgrobiologicalGeneCenter，Shanghai201106，China； 2Collegeof PlantSciences Technology，Huazhong AgriculturalUniversity，Wuhan430070，China；#Theseauthorscontributeequallytothispaper； Correspondingauthor，E-mail： lijun@ sagc．org．cn) ZHENG Xiaoguo，CHEN Liang，LOU Qiaojun，eta1．ChangesinDNA methylationpatterninawater-savingand drought—resistancericevarietyatthree—leafandfour-leafstagesafterdroughtdomestication．ChinJRiceSci，2014，28 (1)：32—4O． Abstract：A water-saving and drought—resistance rice Huhan 3 wasgrown underdroughtstress forsix successive generationsaftertransplantinginthefield．ThevariationsinDNA methylationpatternbetweentheorigina1generation (G。)and thesixthgeneration (G6)wereanalyzed by methylation sensitive amplificationpolymorphism (MSAP) method．Theresultsrevealedthatthemethylatedlociaccountedforabout34 ofthetota1．Amongthese，about84 werefull—methylatedlociandabout16％ werehemi—methylatedones．TheDNA methylationleveldecreasedfrom the three—leafstagetothefour—leafstageinHuhan3．Differentiallymethylated1oci(DML)betweengenerationsor／and betweendifferentdevelopmentalstagesaccounted for4．0 ofthetotal，mostofwhich wereonly related to plant development(57．9 )．ComparedtoG0，theDNA methylationpatterno