2006～2007年流行流感病毒减毒疫苗株的构建.pdfVIP

维普资讯 羹羹 嘲 囫 盈■ I1]l3 … vcwxv．pibb．ac．ca ConstructionofAttenuatedInfluenza VirusVaccinesof2U0U06I6I～～Z2U0U077半’ YANGPeng—Hui)．．，YEyi2)*，SHIXin—Fu)’LUoDe—Yan)’ZHANGY_u YANYan)’zHANGPeng—Feil)， DUANYue—Qiang”，X GLiLIUXiu—Fan)．”，WANGXi—Liang)．” Q~InstituteofMicrobiologyand印idemioolyg,AcademyofMilitaryMedcialScience，StateKeyLaboratoryof PathogenandBoisecurity,Beijing100071，China； CollegeofAnimalScienceandTechnoloyg,NorthwestSci＆TechUniversityofAgricultureandForestry,Yangting712100．Chnia； KeyLbaoratoryforAnimalInfectoiusDiseasesofMinistryofAgriculture，YangzhouUniversity,Yangzhou225009，Chnia) Abstract Sixgenesegments，PB1，PB2，PA，NP，M andNS，werefullysynthesizedwhich derivedfiom htemasterdonorvirus (MDV)，cold-adapted(ca)，temperaturesensitive(ts)，liveattenuatedinfluenzavirusstrainA／AnnArbor6／6／0(MDV-A)．Meanwhile． fiveaminoacidsubstitutions(PB1-391E，581G，66lT，PB2-265S，NP-34G)wereartificiallyalteredbyhumna nitervention．HAand NAframg entsderivedfi-om hte2006~2007circulatnigstraniA／NewCaledonia／209／9fH1N1)．Eightframg entswere ligatedwim modifiedpAD3000forrescueplasmid construction．Eihgttranscription／expressionplasmidswerenamedasPⅡ)、，．A．PB2． pMDV-A-PB1，pMDV-A ·PA，pMDV-A·NP，pMDV-A-M ，pMDV-A-NS，pMDV-A．HA，pMDV-A．NA， respectively．TheCOS．1cells wereco-transfectedwihteihgtplasmidsrepresenting6niternalviralbackboneofhtestrainAA／A／6／60nadtwoplasmidscontaininghte cDNAofhteHAandNA semg entsofhtestrainA／NewCaledonia／20／99 1N1)，hteresultsshowedhtatcold．aadpted，attenuated reassortantnifluenzaA viruswas rescuedsuccessuflly．TitersofareassortedinfluenzaA virusni embryonatedchickeneggsranged