Molecular characterization and expression of HSP70, HSF and HSBP genes in Octopus vulgaris during thermal stress.pdfVIP

Acta0ceanoLSin．，2015，Vo1．34，No．8，P．62-72 DOI：10．1007／0708—6 http：／／www．hyxb．org．cn E-mail：hyxbe@263．net MolecularcharacterizationandexpressionofHSP70，HSFand HSBPgenesinOctopusvulgarisduringthermalstress HONGlingni，MAOYong，NIUSufang，SUNTiantian，SUYongquan CollegeofOceanandEarthSciences，Xiam enUniversity，Xiamen361102，China Received8lune2014；accepted28October2014 ~TheChineseSocietyofOceanographyandSpringer—VerlagBerlinHeidelberg2015 Abstract TemperatureisanimportantenvironmentalfactorthataffectsthegrowthandsurvivalofOctopusvulgaris，the commonoctopus．Tounderstandtheprotectivem echanism thatO．vulgarisexhibitsunderheatstress，weused rapidamplificationofcDNAends(RACE1toobtainfull—lengthsequencesofthreeheatstressresponserelated genes：(1)theheatshockprotein70(OvHSP70)，(2)theheatshocktranscriptionfactor(OvHSF)，and3【)theheat shockfactor—bindingprotein(OvHSBP)of0．vulgaris．TheOvHSP70，OvHSF，andOvHSBPproteinscontained 2222bp，2264bp，841bpthatencodedfor635，458and90am inoacids，respectively．Theresultsofmultiple sequencealignm entshowedhtattheam inoacidsequencesof0vHSp70werehighlyconservedwithrespectto o血erspecies．Similarly，theDNAbindingdomain，thetrimerizationdomainofOvHSF，andthecoiledcoi1region of0vHSBPalsohadhighlyconservedregions．Therea1．timepolymerasechainreaction(PCR)resultsindicated thatOvHSP70wastemperature．dependentandtime．dependent，showingapositiveresponsetoheatstress．On exposureto28。Candto30。C．themRNA expressionlevelsofOvHSFand0vHSBPwerehigherthanthoseinthe controlgroupat24。C．ThemRNA expressionofOvHSBPsignificantlyincreasedwithheattreatmentat26。C，while them RNAexpressionofOvHSFdecreased．Theexperim entalresultsindicatedthattheexpressionof0vHSP70． OvHSFandOvHSBPwereallsensitivetoheatstress．whichsuggeststhathtesethreegenesmayplayanim portant rolefor0．vul~arisinrespondingtoenvironmentalsrtess．Thus，thisstudysetsatheoreticalofundationforfurhter in—depthstudiesonthemolecularprotectivemechanismsofhteheatresponsein0．vulgaris． Kevwords：0ctopusvulgar