可逆识别Hg2+荧光探针的合成及活细胞中成像的应用有机化学.pdfVIP

DOI: 10.6023/cjoc201403009 研究论文 有机化学 Chinese Journal of Organic Chemistry ARTICLE 可逆识别Hg2＋荧光探针的合成及活细胞中成像的应用 周 佳 杨美盼 孟文斐 成 昭 杨秉勤* (合成与天然功能分子化学教育部重点实验室 西北大学化学与材料科学学院 西安 710069) 摘要 以罗丹明B 与邻苯二甲酰氯为原料缩合反应得到新化合物T1, 通过荧光光谱法研究了化合物与金属离子的识别 特性. 结果表明荧光探针 T1 能对 Hg2＋进行快速、选择性、可逆的识别, 且常见金属离子对其干扰较小. 探针的荧光强 度与 Hg2＋浓度(4 ×10－8～9 ×10－8 mol/L)呈现较好的线性关系. 探针在 HepG2 活细胞中的荧光显微成像表明 T1 可应用 于生物体的检测. 关键词 罗丹明 B; 荧光探针; 汞离子; 识别; HepG2 活细胞 Synthesis of a Reversible Fluorescence Probe for Hg2＋ and Its Application in Living Cells Zhou, Jia Yang, Meipan Meng, Wenfei Cheng, Zhao Yang, Bingqin* (Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry Materials Science, Northwest University, Xian 710069) Abstract A new rhodamine B derivative T1 has been rationally designed and synthesized facilely by the condensation reac- tion of rhodamine B hydrazide and o-phthaloyl chloride. The recognition properties of the target compound T1 with metal ions had been investigated by the fluorescence spectrophotometry. This probe exhibits several favorable features, including instan- taneous and reversible recognition, high sensitivity and selectivity to mercury ions. When the concentration of Hg2＋ was in the range of 4 ×10－8～9 ×10－8 mol/L, there was a good linearity between the fluorescence intensity and the concentration of Hg2＋. The fluorescence imaging experiments of Hg2＋ in HepG2 living cells demonstrated their valuable application in biological system. Keywords rhodamine B;