新型烟酰胺腺嘌呤二核苷酸(NAD)类似物的合成及其辅酶活性有机化学.pdfVIP

DOI: 10.6023/cjoc1106032 研究论文 有机化学 Chinese Journal of Organic Chemistry ARTICLE 新型烟酰胺腺嘌呤二核苷酸(NAD)类似物的合成及其辅酶活性 侯淑华a,b 刘武军a 赵宗保*,a (a 中国科学院大连化学物理研究所生物技术部 大连 116023) (b 中国科学院研究生院 北京 100049) 摘要 尝试 4 种形成焦磷酸键的方法合成了 5 种结构新颖的新型烟酰胺腺嘌呤二核苷酸(NAD)类似物. 初步考察了类 似物的生物活性, 发现苹果酸酶和醇脱氢酶以类似物 3b 和 3d 为辅酶时, 活性只有以 NAD 为辅酶时的 13%～30%; 而 以类似物3a, 3c 和 3e 为辅酶时, 这些酶的活性均极低. 关键词 NAD 类似物; 焦磷酸; 生物活性; 醇脱氢酶 Synthesis of Novel Nicotinamide Adenine Dinucleotide (NAD) Analogs and Their Coenzyme Activities Hou, Shuhuaa,b Liu, Wujuna Zhao, Zongbao*,a a ( Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023) (b Graduate University of Chinese Academy of Sciences, Beijing 100049) Abstract Four methods were tested to synthesize nicotinamide adenine dinucleotide (NAD) analogs, and 5 NAD analogs were prepared. Assays of these NAD analogs indicated that malic enzyme and alcohol dehydrogenase had about 13%～30% enzymatic activity in the presence of analog 3b or 3d, whereas significantly lower activities were observed in the presence of analog 3a, 3c or 3e. Keywords NAD analog; pyrophosphate; bioactivity; alcohol dehydrogenase 烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinu- 是生物体内不可缺少的小分子化合物. NAD 依赖型生物 cleotide, NAD) 以及它相应的还原型态(NADH, 图 1), 大分子在生命活动中具有非常重要的作用, 参与能量传 O