番鸭就巢性状相关基因表达的cDNA-AFLP分析.pdfVIP

中国农业科学 2012,45(2):353-358 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.02.018 番鸭就巢性状相关基因表达的cDNA-AFLP分析 吴 旭, 严美姣, 刘丽平, 傅星源, 连森阳, 李 昂 （福建农林大学动物科学学院，福州 350002 ） 摘要：【目的】寻找与番鸭就巢性状相关的表达基因，了解其就巢调控的分子机理。【方法】采用 cDNA-AFLP (cDNA-amplified fragment length polymorphism)技术分析遗传背景相近的 RF 系白羽番鸭就巢个体和非就巢个 体脑垂体基因表达差异。【结果】筛选到与就巢行为相关差异表达片段82条，选取其中15条进行测序。将测序结 果在 GenBank 数据库进行 BLAST 比较和分析，发现 8 个片段(53.3%)与已知基因具有较高同源性，涉及与繁殖性 能紧密相关的下丘脑-垂体-性腺轴(hypothalamic-pituitary-gonadal，HPG)上的功能基因，如 GnRH 基因、FSH 基因，同时也分离到位于GH／IGF轴上的功能基因，即GH 基因；以及在功能上属于信号传导、转录调控、代谢的 调节基因，如超氧化物歧化酶基因、角蛋白关联蛋白基因、硫氰酸酶释放硫基转移酶基因等。2 个片段与未知功 能的基因有同源性，5个与数据库中现有的基因没有明显的相似性。随机选择3个片段进行RT-PCR 验证，检测片 段的表达模式与cDNA-AFLP 结果一致。【结论】揭示番鸭不同就巢性状基因表达调控模式，进一步阐明番鸭就巢的 分子机理。 关键词：番鸭; 就巢, cDNA-AFLP; 表达谱; 差异表达基因 cDNA-AFLP Analysis on Transcripts Associated Gene with Broodiness in Muscovy Duck WU Xu, YAN Mei-jiao, LIU Li-ping, FU Xing-yuan, LIAN Sen-yang, LI Ang (College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002) Abstract: 【Objective 】 The objective of this experiment is to identify differentially expressed genes of Muscovy Duck broodiness. 【Method 】cDNA-AFLP approach was employed to analyze genes differentially expressed in broodiness and non-broodiness Muscovy Duck individuals derived from a single RF white feathers line. 【Result 】 There were 82 differentially expressed bands associated with broodiness behaviors, 15 bands of them were subject to sequence analysis. Database searches indicate that 8 cDNA fragments show high homology to known genes, which include some function genes