高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响.pdfVIP

中国农业科学 2007,40(8):1622-1629 Scientia Agricultura Sinica 高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响 李木英，石庆华，胡志红，潘晓华，谭雪明 （江西农业大学农学院/江西省作物生理生态与遗传育种重点实验室，南昌 330045 ） 摘要：【目的】探讨高温胁迫对水稻胚乳灌浆影响的酶学生理特征。【方法】选用水稻耐热型品种 082、农大 228和热敏感品种协青早B、茉莉占，采用高温胁迫与常温对比试验方法，研究灌浆前期高温对胚乳淀粉合成酶类 活性及淀粉积累的影响。【结果】胚乳淀粉合成酶活性高峰出现在花后9～12 d。高峰期间及之前，胚乳SS、AGPase、 SSS 和 Q 酶受高温影响活性上升，高峰之后，除 Q 酶外，均较对照下降，耐热品种胚乳淀粉合成酶类活性的下降 幅度明显小于热敏感品种。高温影响淀粉积累速率与 AGPase、SSS、SS 和 R 酶活性相关显著。【结论】不同水稻 品种胚乳淀粉合成酶对高温的感应存在差异，AGPase、SSS、SS 和 R 酶是高温影响淀粉积累速率的关键酶。高温 影响直链淀粉含量下降与Q 酶活性不存在必然的联系。 关键词：早稻；高温胁迫；胚乳；酶活性 Effects of High Temperature Stress on Activity of Amylosynthease in Endosperm of Early Indica Rice Varieties LI Mu-ying, SHI Qing-hua, HU Zhi-hong, PAN Xiao-hua, TAN Xue-ming (College of Agronomy, Jiangxi Agricultural University/Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province, Nanchang 330045) Abstract: 【Objective 】Physiological mechanisms of the impact of high temperature stress on rice endosperm filling were discussed. 【Method 】 On the basis of the field controlled canopy, four early indica rice cultivars with different heat sensitivities, namely Nongda228 and 082 (heat-resistant) and Molizhan and XeiqingzaoB (heat–sensitive), were applied to compare their difference in response of some enzymes involved in starch synthesis to high temperature stress at early filling stage. 【Result 】The results indicated that the active peak of amylosynthease in endosperm appeared in 9-12 day after anthesis. Affected by high temperature, before and during the period of the peak, the activities of SS, AGPase, SSS and Q enzymes increased. After that, these activities decreased except for Q enzyme. The decreasing extent in heat resis