利用基因芯片研究小麦耐盐突变体盐胁迫条件下基因的表达图谱.pdfVIP

中国农业科学 2007,40(10):2355-2360 Scientia Agricultura Sinica 利用基因芯片研究小麦耐盐突变体盐胁迫条件下 基因的表达图谱 赵宝存，赵 芊，葛荣朝，沈银柱，黄占景 （河北师范大学生命科学学院，石家庄 050016 ） 摘要：【目的】研究小麦在不同盐胁迫时间下根部基因的应答反应。【方法】利用基因芯片技术，分析盐胁迫 下耐盐小麦RH8706－49的小麦根部基因的表达情况。【结果】获得了61 215个小麦基因的差异表达图谱。在不同 盐胁迫时间下大量根部基因的表达发生很大变化，即有盐诱导表达的基因，也有盐抑制表达的基因，同时对杂交 数据进行多种聚类分析，并对基因表达差异的原因进行了初步分析。【结论】小麦耐盐机理非常复杂，是大量基因 协调表达的结果，其中盐诱导表达基因的作用非常重要。 关键词：基因芯片；小麦；耐盐基因；盐胁迫；盐诱导基因 Study on the Expression Profile of Salt-Tolerance Mutant Under Salt-Stress in Wheat Using Gene Microarray ZHAO Bao-cun, ZHAO Qian, GE Rong-chao, SHEN Yin-zhu, HUANG Zhan-jing (College of Life Science, Hebei Normal University, Shijiazhuang 050016) Abstract: 【Objective 】The objective of this experiment is to study the reactions of different genes of root to salt-stress in wheat. 【Method 】The expression of genes in root of wheat was studied by using gene chip technology. 【Result 】 The expression of all genes in root of wheat salt-tolerance mutant RH8706-49 were analyzed using gene chip technology. The responses to different times of salt stress were studied. The differential expression profile of 61215 genes was gained. The clustering analysis of hybridization data showed that gene expression changed greatly in root under salt stress. Both salt-induced genes and salt-inhibited genes were found. 【Conclusion 】The mechanism of salt-tolerance in wheat is very complicated and it is the result of a lot of genes expression in line. The function of the salt-induced genes is very important. Key words: Gene chip; Wheat; Salt stress; Salt-tolerance gene; Salt-induced gene 克隆了耐盐相关基因[6] ，但是，耐盐是一个数量性状， 0 引言 相关基