连翘酯苷对鸡IFN-alpha;和JAK-STAT信号通路相关因子的影响.pdfVIP

中国农业科学 2011,44(18):3903-3908 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.18.022 连翘酯苷对鸡 IFN- α和JAK-STAT 信号通路相关因子的影响 李华伟, 张羽璐, 张中文, 夏 楠, 郭 玮, 吴国娟 （北京农学院动物科学技术学院，北京 102206 ） 摘要：【目的】探讨连翘酯苷作用于鸡胚肾（CEK）细胞后，IFN- α的表达变化及JAK-STAT通路相关因子mRNA 的表达变化，及连翘酯苷对IFN- α和JAK-STAT信号通路的影响。【方法】将连翘酯苷设为 3 个浓度（100、200 和 400 μg·mL-1），采用荧光定量RT-PCR，检测IFN- α和JAK-STAT通路相关因子的mRNA表达变化；用western blotting 检测IFN- α的蛋白表达情况。【结果】与正常组相比，连翘酯苷不仅显著提高了IFN- α的表达量（P＜0.05）；而且 明显上调了STAT1、JAK1、IFNAR1、IFNAR2、IRF1、IRF7 的表达。【结论】连翘酯苷能够在鸡胚肾细胞（chicken embryo kidney cells，CEKs） 上诱导IFN- α的表达，且能够正向调节JAK-STAT信号通路传导。 关键词：连翘酯苷; 鸡胚肾细胞; IFN- α; JAK-STAT; real-time PCR; western blotting Forsythiaside A Induced IFN-α and Up-Regulated Correlation Factors of JAK-STAT Signaling Pathways in vitro LI Hua-wei, ZHANG Yu-lu, ZHANG Zhong-wen, XIA Nan, GUO Wei, WU Guo-juan (College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206) Abstract: 【Objective 】This study focused on the effect of forsythiaside A added in chick embryo kidney cells, the expression of IFN- αand the mRNA expression of correlation factors in JAK-STAT pathway. 【Methods 】In the study, three levels forsythiaside (100, 200, 400 μg·mL-1) were set, and real-time RT-PCR was used to detect the mRNA expression of IFN-α and JAK-STAT pathway related factors; western blotting was used to investigate protein expression of IFN-α. 【Result 】 Compared with normal group, the expression of IFN-α increased significantly when high dose forsythiaside A was added (P ＜0.05). And the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1, IRF7 was significantly increased either. 【Conclusion 】Forsythiaside A induced the expression of IFN-α in chicken embryo kidney cells (CEKs), and can posit