利用单片段代换系定位水稻粒形QTL.pdfVIP

中国农业科学 2006,39(4):647-654 Scientia Agricultura Sinica 利用单片段代换系定位水稻粒形QTL 曾瑞珍，Akshay TALUKDAR ，刘 芳，张桂权 (华南农业大学／广东省植物分子育种重点实验室，广州 510642) 摘要：【目的】水稻谷粒形状（粒长、粒宽和长宽比）是衡量稻米外观品质的重要指标之一，为更好地开展 粒形分子育种，对水稻粒形QTL进行分子定位。【方法】以单片段代换系（SSSL）为材料构建分离群体，利用微卫 星标记对控制水稻谷粒长和谷粒宽的 2 个粒形 QTL 进行分子定位。【结果】粒宽 QTL Gw-8 被定位于第 8 染色体长 臂末端微卫星标记RM502与RM447之间, 遗传距离均为0.3 cM。在此基础上构建了覆盖 Gw-8 的物理图谱，RM502 与RM447位于同一克隆AP005529，两者之间的物理距离为55.0 kb。粒长QTL gl-3 被定位于第3染色体着丝粒附 近的微卫星标记 RM6146 和 PSM377 之间，遗传距离分别为 1.5 cM 和 11.0 cM。【结论】利用单片段代换系能准确 地定位水稻粒形QTL，这两个粒形QTL的定位为其克隆及稻米外观品质的分子育种奠定了基础。 关键词：水稻；基因定位；粒形；单片段代换系 Mapping of the QTLs for Grain Shape Using Single Segment Substitution Lines in Rice ZENG Rui-zhen, Akshay TALUKDAR, LIU Fang, ZHANG Gui-quan (Guangdong Key Laboratory of Plant Molecular Breeding / South China Agricultural University, Guangzhou 510642 ) Abstract ：【Objective 】Grain shape (grain length, grain width and length-width ratio) is a main index of rice appearance quality. For molecular breeding in rice, two QTLs controlling grain shape were mapped. 【Method 】The mapping populations were developed from the single segment substitution lines (SSSLs) in indica background of Huajingxian 74 and the two QTLs of grain shape were mapped using SSR markers. 【Results 】The grain width QTL, Gw-8, was mapped on the long arm of chromosome 8 between RM502 and RM447 with genetic distance of 0.3 cM. RM502 and RM447 located on the same clone AP005529 and the physical distance between RM502 and RM447 was 55.0 kb. The grain length QTL, gl-3, was located near the centromeric region of rice chromosome 3 between RM6146 and PSM377 with genetic distanc