噬菌体展示三肽素（bursin）模拟肽的研究.pdf

江西农业大学硕士论文 摘 要 J 序列，并与Bursin序列进行同源性分析，通过ELISA法检测其结合活性和 鸡体内的生物学效应。序列分析表明，“LNXT”短肽序列可能为结合抗 抗体(2F9．4／HU2)的结合有一定的抑制作用；生物学活性初步验证表明， 阳性克隆(No．4)能促进抗体产生与囊素具有类似作用，有望作为一种模 拟Bursin的新型的免疫增强剂。 关键词：囊素；噬菌体展示：模拟肽 江西农业大学硕士论文 Abstract Anti—Bursinmonoclonal antibody(2F9—4／HU2)waspurified．The was withanti—Bursinmonoclonal biopanned peptide from ssDNAofthe clones display 1ibrary．The were phage peptide positive the between and binding phage anti—Bursin sequenced，and displaypeptide monoclonal evaluated was antibody(2F9—4／HU2)wasbyELISA．Antigencity with ELISA．In to was competitireaddition，contrast assayed Bursin，test to conducteddetectthe ofthe bioactivity clone(No．4)inchick positive istheconservative with “LNXT”perhaps sequence anti-Bursin binding monoclonal isnot to Bursin．Positive antibody(2F9—4／HU2)。which isogenic is to clone(No，1)containsK、H、G．which Bursin．ELISAand isogenic ELISAdescrib