通便玉蓉丸制剂艺与通便机制的研究.pdfVIP

中文摘要 目的：观察不同制剂工艺的通便玉蓉丸对小鼠便秘模型通便的效应，探究通便玉 蓉丸对小鼠便秘模型的作用机制，用中医药理论阐释通便玉蓉丸治疗肾虚气血不和所 致之便秘立法依据和组方原理，阐述补肾益精养血而达到润肠通便功效的科学内涵。 方法：实验研究采用地芬诺酯灌胃复制小鼠便秘模型，小肠营养糊推进实验观察 不同工艺通便玉蓉胶囊对正常小鼠和便秘模型小鼠肠运动的影响；胃内营养糊残留实 验观察不同制剂工艺通便玉蓉胶囊对便秘模型小鼠胃排空的影响；酶联免疫法检测便 秘模型小鼠小肠中血管活性肠肽（VIP ）的水平；HE 染色法观察便秘模型小鼠空肠 段粘膜病理形态改变。 结果：通便玉蓉丸醇提制剂工艺和水提制剂工艺能够提高正常小鼠小肠推进率， 与对照组相比有显著差异性（p0.01 ）；通便玉蓉丸醇提制剂工艺和水提制剂工艺能 够提高便秘模型小鼠小肠推进率，与对照组相比有显著差异性（p0.05 或p0.01 ）； 通便玉蓉丸醇提制剂工艺，和水提制剂工艺能够提高便秘模型小鼠胃排空，与对照组 相比有显著差异性（p0.05 或 p0.01 ）；通便玉蓉丸醇提制剂工艺和水提制剂工艺 有提高便秘模型小鼠小肠组织血管活性肠肽（VIP ）水平的趋势；与对照组相比有显 著差异性（p0.05 或p0.01 ）；各便秘模型组小鼠空肠段黏膜层完整，均未见充血、 水肿及炎细胞浸润。 结论：通便玉蓉丸可增强正常小鼠、便秘模型小鼠的小肠蠕动；并且能增强便秘 模型小鼠的胃排空运动；促进小鼠肠道内VIP 的释放；醇提、水提制剂工艺的通便玉 蓉丸改善便秘症状，可能是其温肾益精、润肠通便功效的依据之一。 关键字：通便玉蓉丸；便秘；小肠肠推进；胃排空；VIP Research of Preparation and Mechanism of TongBianyurong Pill ABSTRACT Objective: To observe the effect of different preparations of TongBianyurong pill on gastrointestinal peristalsis of constipation model mice,to explore the mechanism of different preparations of TongBianyurong pill.On the guidiance of theory of Chinese medicine to interpretate the accordance and principle of formulation of TongBianyurong pill.To state the connotation of the effect of TongBianyurong pill which can loosen bowel by warming kidney,nourishing essence and regulating the relationship between qi and blood. Methords: The effect of different preparations of TongBianyurong pill on gastrointestinal peristalsis of normal health mice was observed by Evans blue nutrition paste propelling test. The effect of different preparations of TongBianyurong pill on gastrointestinal peristalsis of constipation model mice was observed by Evans blue nutrition paste pro