河西走廊盐碱土壤中抗立枯丝核菌的放线菌筛选.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2014 1 河西走廊盐碱土壤 中抗立枯丝核菌的放线菌筛选 王蓓  牛世全  达文燕  李海云  胡娇龙  赵国杰 （西北师范大学生命科学学院 旱区微生物资源与生态研究所，兰州 730070） 摘 要 ： 从甘肃省河西走廊盐碱土中分离所得50 株放线菌中，筛选出对立枯丝核菌有生防潜能的放线菌，旨在为生物防治 作物病害提供新的菌种资源。通过平板对峙法、生长速率法及活体组织法筛选拮抗菌，并对其进行形态及分子鉴定。经过平板初筛， 有4 株菌株对立枯丝核菌有拮抗作用，编号Ⅰ18-5-2、Ⅲ22-3-12 、Ⅳ16-3-2 、DC009-1-23 ，其中Ⅳ16-3-2 和DC009-1-23 有较高的 拮抗性，抑菌圈直径分别为20.5 mm 和15.5 mm ；进而对这2 菌株进行发酵液复筛，菌丝的生长抑制率分别为89.02% 和80.49% ； 活体组织试验表明，Ⅳ16-3-2 和DC009-1-23 对立枯丝核菌的防治效果分别达54.29% 和45.7 1% ；通过形态培养特征和16S rDNA 序列鉴定，将菌株Ⅳ16-3-2 初步鉴定为变异链霉菌，DC009-1-23 为丁香链霉菌。 关键词 ： 立枯丝核菌 放线菌 盐碱地 生物防治 Screening of Actinomyces on Antagonism to Rhizoctonia solani Isolated - from Saline alkali Soils in Hexi Corridor Wang Bei Niu Shiquan Da Wenyan Li Haiyun Hu Jiaolong Zhao Guojie （Institude of Arid Microbial Resources and Ecology ，College of Lif e Science ，Northwest Normal University ，Lanzhou 730070 ） Abstract:  This study selected several actinomycetes from 50 strains of the saline-alkali soils of Hexi Corridor in Gansu Province which have been proved to be resistant to Rhizoctonia solani by antagonistic test, which would provide new strains resources in crop disease control. Pair culture method, growth rate method and tissue culture method were used to select antagonistic bacteria. The results of antagonistic test showed that there were 4 strains with antagonism to Rhizoctonia solani, which were named Ⅰ18-5-2, Ⅲ 22-3-12, Ⅳ 16-3-2 and DC009-1-23. From above four strains, Ⅳ 16-3-2 and DC009-1-23 had a higher antagonism and the diame