番茄黄萎病病原菌鉴定和抗病种质资源筛选.pdfVIP

IDENⅡFICATl0N oF THE P椰oGENS OF Verticillium WIIT ON T0脚O AND SCREENING OF RESISTANT GERⅣⅢLASM ABSTRACT Ver“cillhan dahiea wilt of tomato was one of serious diseases which happened both in field and greenhouse in many countries ofthe world．In recent years，this disease appeared the increasingly aggravative tendency and sharply spreading tendency in the tomato production in our country．The selection of disere．resistant varieties is more economic and effective method．In this test,pathogen of tomato Verticillium dahiea wilt was identified and physiology characteristic of pathogen was studied．n”race differentiation was determined． At the saxale time．the study foand∞easy and simple resistant identifieation method in seedling stage and carried on resistant identification of Ferticillium dahlea wilt to tomato germptasm reSOUPCeS that are kept by our lab．Offered the theoretical foundation for Verticillium dahiea wilt prevent and cure in protective tomato production and tomato resistant disease breeding of他rlicillivan dahiea wilt． The result of study indicated：the isolate that was obtained in the test was pathogen causing tomato阮rticillim wilt,and it was Verticillium dahiea ldeb． Biological charac钯ristic research indicated：the culture mediums that were suitable for tomato Verticillium．dahiea were PSA culture medium，low quantity sucrose yeast culture medium potato carrot culture medium and Cai cultore mcdium，pctato carrot culture medium was most ideal for producing spore． The most suitable temperature for hypha growth was 22．5℃，the optimal temperature for spore germination was 25℃．optimal pH for hypha growth w∞5．According to reaction of the identification hosts and stains．it call be certained that VerticilliⅢdahliae of tomato that be isolated and used in the钯st was race l， The paper screened the method of resistance identification for Irerticilliura dahliae of tomato in seedling stage．the best combination was immersing root with 10+spores／ml during 2 leaves stage The result of